Mouse IL-2 Precoated ELISPOT Kit

- Overview
  - The kit includes detection antibody, streptavidin-HRP, TMB Membrane Substrate, dilution buffer, Cell stimulator , washing buffer and multifilter plate that is pre-coated with captured antibody. The kit is a ready-to-use and quality assurance product for all needs of ELISPOT assay. The performance of the kit is carefully examined to other comparable ELISPOT kits world wide to assure equivalent sensitivity, stability, and reproducibility.
    
    Please contact us at for specific academic pricing.
- Properties
  - Details
    
    Biotinylated antibody: 100μL
    Streptavidin-HRP: 100μL
    Cell stimulator: 50T
    Dilution buffer R (1×): 10mL×2
    Washing buffer (50×): 15mL
    TMB Membrane Substrate: 10mL
    Precoated PVDF plate: 1 plate
    
    Storage
    
    Store the unopened kit at 2-8°C. The kit is stable for 12 months. Do not use beyond expiration. Keep sterile once opened.
    
    * For Research Use Only.
- Reference
  - Liu,et al.[J] Chinese Journal of Immunology,2005,21(5):367-368
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    Zhou,et al.[J] Chinese Journal of Immunology,2007,12
    Xiao,et al.[J] Journal of Cellular and Molecular Immunology, 2007, 23(12): 1130-1132,1135
    Xu,et al.[J] Chinese Journal of Bilogicals, 2007,20(12): 907-909
    Dong,et al.[J] Journal of Modern Food and Pharmaceuticals,2007,17(4):3-5
    Liu,et al.[J] Chinese Journal of Pathophysiology,2007,23(1):99-102
    Yuan,et al.[J] Chinese Pharmaceutical Journal,2007,42(13):998-1002
    Zhang,et al.[J] Chinese Journal of Microbiology and Immunology,2007 ;27(11):1059-1060
    Xian-Hua Wang, et,al.[J]World J Gastroenterol,2005;11(36):5614-5620
    Xu-Dong Tang, et,al.[J]Cancer Research,2008,68(5):1529–1537
    Ting Chen,Xu-Dong Tang, et,al. [J] Neoplasia;2008(10):977–986.
    Ling Chen,Xu-Dong Tang,et,al. [J] J Pathol. 2009 Apr; 217(5):685-92.
    Zorn E, Nelson E A, Mohseni M, et al. IL-2 regulates FOXP3 expression in human CD4+CD25+ regulatory T cells through a STAT-dependent mechanism and induces the expansion of these cells in vivo[J]. Blood, 2006, 108(5):1571-1579.
    Kambayashi T, Assarsson E, Chambers B J, et al. IL-2 down-regulates the expression of TCR and TCR-associated surface molecules on CD8(+) T cells.[J]. European Journal of Immunology, 2015, 31(11):3248-3254.
    Mitra S, Leonard W J. Biology of IL-2 and its therapeutic modulation: Mechanisms and strategies[J]. Journal of Leukocyte Biology, 2018, 103(4):643-650.
    Chinen T, Kannan A K, Levine A G, et al. An essential role for the IL-2 receptor in Treg cell function[J]. Nature Immunology, 2016, 17:1322-1333.
    Bayer A L, Pugliese A, Malek T R. The IL-2/IL-2R system: From basic science to therapeutic applications to enhance immune regulation[J]. Immunologic Research, 2013, 57(1-3).
    Wrangle J M, Patterson A, Johnson C B, et al. IL-2 and Beyond in Cancer Immunotherapy[J]. Journal of Interferon & Cytokine Research, 2018, 38(2):45-68.

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Mouse IL-2 Precoated ELISPOT Kit

Mouse IL-2 Precoated ELISPOT Kit

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