Human IL-4 Precoated ELISPOT Kit

- Overview
  - The kit includes detection antibody, streptavidin-HRP, TMB Membrane Substrate, dilution buffer, Cell stimulator , washing buffer and multifilter plate that is pre-coated with captured antibody. The kit is a ready-to-use and quality assurance product for all needs of ELISPOT assay. The performance of the kit is carefully examined to other comparable ELISPOT kits world wide to assure equivalent sensitivity, stability, and reproducibility.
    
    Please contact us at for specific academic pricing.
- Properties
  - Details
    
    Biotinylated antibody: 100μL
    Streptavidin-HRP: 100μL
    Cell stimulator: 50T
    Dilution buffer R (1×): 10mL×2
    Washing buffer (50×): 15mL
    AEC dilution: 10mL
    AEC solutionⅠ (20×): 500μL
    AEC solutionⅡ (20×): 500μL
    AEC solution Ⅲ (200×): 50μL
    Precoated PVDF plate: 1 plate
    
    Storage
    
    Store the unopened kit at 2-8°C. The kit is stable for 12 months. Do not use beyond expiration. Keep sterile once opened.
    
    * For Research Use Only.
- Reference
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    Zhou,et al.[J] Chinese Journal of Immunology,2007,12
    Xiao,et al.[J] Journal of Cellular and Molecular Immunology, 2007, 23(12): 1130-1132,1135
    Xu,et al.[J] Chinese Journal of Bilogicals, 2007,20(12): 907-909
    Dong,et al.[J] Journal of Modern Food and Pharmaceuticals,2007,17(4):3-5
    Liu,et al.[J] Chinese Journal of Pathophysiology,2007,23(1):99-102
    Yuan,et al.[J] Chinese Pharmaceutical Journal,2007,42(13):998-1002
    Zhang,et al.[J] Chinese Journal of Microbiology and Immunology,2007 ;27(11):1059-1060
    Xian-Hua Wang, et,al.[J]World J Gastroenterol,2005;11(36):5614-5620
    Xu-Dong Tang, et,al.[J]Cancer Research,2008,68(5):1529–1537
    Ting Chen,Xu-Dong Tang, et,al. [J] Neoplasia;2008(10):977–986.
    Ling Chen,Xu-Dong Tang,et,al. [J] J Pathol. 2009 Apr; 217(5):685-92.
    Dardalhon V , Awasthi A , Kwon H , et al. IL-4 inhibits TGF-beta-induced Foxp3+ T cells and, together with TGF-beta, generates IL-9+ IL-10+ Foxp3(-) effector T cells.[J]. Nature Immunology, 2008, 131(12):1347-1355.
    Tahani S, Dehghani L, Jahanbani-Ardakani H, et al. Elevated serum level of IL-4 in neuromyelitis optica and multiple sclerosis patients[J]. Journal of Immunoassay and Immunochemistry, 2019, 40(3):1-9.
    Bosurgi L, Cao Y G, Cabeza-Cabrerizo M, et al. Macrophage function in tissue repair and remodeling requires IL-4 or IL-13 with apoptotic cells[J]. Science, 2017, 356(6342):1072-1076.
    Gessner A, Mohrs K, Mohrs M. Mast Cells, Basophils, and Eosinophils Acquire Constitutive IL-4 and IL-13 Transcripts during Lineage Differentiation That Are Sufficient for Rapid Cytokine Production[J]. The Journal of Immunology, 2005, 174(2):1063-1072.
    Black S M, Schott M E, Batdorf B H, et al. IL-4 induces protection of vascular endothelial cells against killing by complement and melittin through lipid biosynthesis[J]. European Journal of Immunology, 2010, 40(3):803-816.

MSDS

Human IL-4 Precoated ELISPOT Kit

Human IL-4 Precoated ELISPOT Kit

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