Mouse IFN-γ T Cell ELISPOT Kit

Mouse IFN-γ T Cell ELISPOT Kit

Catalog Number:
EK02494196UCY
Mfr. No.:
CT317-PR2/PR5
Price:
$754
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      • Overview
        • The T cell ELISPOT assay is a simple and sensitive immunoassay for the detection of protein secreting T cells at the single cell level.

          Staining: Enzymatic
          Characteristics: PVDF

          Please contact us at for specific academic pricing.

      • Properties
        • Details
          • Coating antibodies
          • Biotinylated detection antibodies
          • Streptavidin-Horseradish Peroxidase
          • AEC coloring system
          • Blocking stock solution
          • Dilution buffer R
          • Tween-20
          • Detailed manual

          * For research use only.

      • Applications
        • Application Description
          The ELISPOT assay is one of the most sensitive assays to monitor ex vivo cellular immune responses at the single cell level. The assay can accurately detect secreted proteins, such as cytokines, released by T cells in response to an antigen. The cell suspensions used in the test can be from blood (PBMC), lymphoid, spleen, bone marrow or CNS tissue.
      • Reference
        • • Vaccination of mice with recombinant baculovirus expressing spike or nucleocapsid protein of SARS-like coronavirus generates humoral and cellular immune responses.
          Bai, Bingke, et al. Mol. Immunol. 2008 Feb;45: 868-75
          • Gene therapy for tolerance: high-level expression of donor major histocompatibility complex in the liver overcomes naive and memory alloresponses to skin grafts.
          Cunningham, Eithne C, et al. Transplantation 2013 Jan 15;95: 70-7
          • The preparation of human papillomavirus type 58 vaccine and exploring its biological activity and immunogenicity in vitro.
          Deng, Dongrui, et al. Int. J. Gynecol. Cancer 2011 Aug;21: 988-95
          • Phenotype and fate of liver-resident CD8 T cells during acute and chronic hepacivirus infection.
          Dravid, Piyush, et al. PLoS Pathog 2023 Oct;19(10): e1011697
          • Identification of cytotoxic T lymphocyte epitopes in dengue virus serotype 1.
          Duan, Zhiliang, et al. J. Med. Virol. 2015 Jul;87: 1077-89
          • Development of a more efficient hepatitis B virus vaccine by targeting hepatitis B virus preS to dendritic cells.
          Jing, Mingzhen, et al. Vaccine 2016 Jan 20;34(4): 516-522
          • Synthetic peptides containing B- and T-cell epitope of dengue virus-2 E domain III provoked B- and T-cell responses.
          Li, Shanfeng, et al. Vaccine 2011 May 09;29: 3695-702
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          Mao, Ruifeng, et al. Drug Deliv 2023 Jan;30(1): 2173339
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          Mirandola, Leonardo, et al. J Transl Med 2019 Dec 19;17: 424
          • STING activator 2'3'-cGAMP enhanced HSV-1-based oncolytic viral therapy.
          Sibal, Patricia Angela, et al. Mol Oncol 2024 Feb 23;: Online ahead of print
          • The self-peptide repertoire plays a critical role in transplant tolerance induction.
          Son, Eric T, et al. J Clin Invest 2021 Nov 01;131(21): e146771
          • Increased antigen presentation but impaired T cells priming after upregulation of interferon-beta induced by lipopolysaccharides is mediated by upregulation of B7H1 and GITRL.
          Wang, Fang, et al. PLoS ONE 2014;9: e105636
          • B21 DNA vaccine expressing ag85b, rv2029c, and rv1738 confers a robust therapeutic effect against latent infection.
          Weng, Shufeng, et al. Front Immunol 2022;13: 1025931
          • Chimaeric protein improved immunogenicity compared with fusion protein of Ag85B and ESAT-6 antigens of Mycobacterium tuberculosis.
          Xu, Y, et al. Scand. J. Immunol. 2006 Nov;64: 476-81
          • Key role of the GITR/GITRLigand pathway in the development of murine autoimmune diabetes: a potential therapeutic target.
          You, Sylvaine, et al. PLoS ONE 2009 Nov 20;4: e7848
          • Down selecting adjuvanted vaccine formulations: a comparative method for harmonized evaluation.
          Younis, Sumera Y, et al. BMC Immunol. 2018 Jan 31;19: 6
          • Ex vivo IL-15 replenishment augments bone marrow precursor cell-mediated adaptive immunity via PI3K-Akt pathway.
          Zhang, Li Xiao, et al. J. Leukoc. Biol. 2020 Jul;108(1): 177-188

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