Name: Mouse anti Desmin, conjugated to FITC
Price: 518.00 USD

- Overview
  - Each vial contains 1ml FITC-conjugated anti desmin monoclonal antibody in PBS containing 0.1% BSA, 0.09% sodium azide. Approximately 100 tests.
    
    Please contact us at for specific academic pricing.
    
    Background
    
    Desmin is a 53 kDa intermediate filament protein that exhibits a high degree of tissue specificity, its expression being predominantly confined to all types of muscle cells (cardiac, skeletal and smooth muscle). Regulation of desmin expression is stage and tissue-specific, since it is induced during terminal development of, for example, skeletal muscle cell differentiation. In skeletal and cardiac muscle cells desmin is localized in the Z-disk region and at the intercalated disk. The expression pattern of desmin in smooth muscle is much more heterogenous. Co-expression of vimentin and desmin has been observed in tumors derived from muscle tissue, i.e. rhabdomyosarcomas and leiomyosarcomas. Furthermore, during myocard dysfunction dramatic changes in the distribution of desmin have been observed.
- Properties
  - Host
    
    mouse
    
    Isotype
    
    IgG2b
    
    Reactivity
    
    chicken; dog; hamster; human; mouse; rabbit; rat; swine; zebrafish
    
    Clone
    
    RD301
    
    Conjugate
    
    FITC
    
    Formulation
    
    Each vial contains 1ml FITC-conjugated anti desmin monoclonal antibody in PBS containing 0.1% BSA, 0.09% sodium azide. Approximately 100 tests.
    
    Storage
    
    The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
    
    Note
    
    This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
    
    * This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
- Applications
  - Application
    
    ICC; IHC (frozen)
    
    Application Description
    
    RD301 is suitable for immunocytochemistry on permeabilized cells and immunohistochemistry on frozen sections.
    
    Optimal antibody dilutions for the different applications should be determined by titration. The recommended dilution is 1:10.
- Reference
  - 1. Quax, W., van den Broek, L., Egberts, W. V., Ramaekers, F., and Bloemendal, H. (1985). Characterization of the Hamster desmin gene: expression and formation of desmin filaments in nonmuscle cells after gene transfer, Cell 43:327-38.
    
    2. Verhagen, A. P., Aalders, T. W., Ramaekers, F. C., Debruyne, F. M., and Schalken, J. A. (1988). Differential expression of Keratins in the basal and luminal compartments of Rat prostatic epithelium during degeneration and regeneration, Prostate 13:25-38.
    
    3. Pieper, F. R., Schaart, G., Krimpenfort, P. J., Henderik, J. B., Moshage, H. J., van de Kemp, A., Ramaekers, F. C., Berns, A., and Bloemendal, H. (1989). Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells, J Cell Biol 108:1009-24.
    4. Schaart, G., Viebahn, C., Langmann, W., and Ramaekers, F. (1989). Desmin and titin expression in early postimplantation Mouse embryos, Development 107:585-96.
    
    5. Raats, J. M., Pieper, F. R., Vree Egberts, W. T., Verrijp, K. N., Ramaekers, F. C., and Bloemendal, H. (1990). Assembly of amino-terminally deleted desmin in vimentin-free cells, J Cell Biol 111:1971-85.
    6. Schaart, G., Pieper, F. R., Kuijpers, H. J., Bloemendal, H., and Ramaekers, F. C. (1991). Baby Hamster kidney (BHK-21/C13) cells can express striated muscle type proteins, Differentiation 46:105-15.
    7. Dispersyn, G. D., Geuens, E., Ver Donck, L., Ramaekers, F. C., and Borgers, M. (2001). Adult Rabbit cardiomyocytes undergo hibernation-like dedifferentiation when co-cultured with cardiac fibroblasts, Cardiovasc Res 51:230-40.
    
    8. Council, L., Hameed, O. (2009). Differential expression of immunohistochemical markers in bladder smooth muscle and myofibroblasts, and the potential utility of desmin, smoothelin, and vimentin in staging of bladder carcinoma Modern Pathology 22:639-50.

Datasheet

Mouse anti Desmin, conjugated to FITC

Mouse anti Desmin, conjugated to FITC

Background

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