Anti-AAV VP1/VP2/VP3 mouse monoclonal, B1, AFDyeTM 488 Conjugate

Overview
- • mouse monoclonal
  • AFDyeTM 488 conjugate
  • suitable for western blot analysis
  • reacts with free VP1, VP2, VP3 proteins of AAV1, 2, 3, 5, 6, 7, 8, 9, rh10, DJ
  • isotype: IgG1
  
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  Background
  
  The B1 antibody reacts with free VP1, VP2 and VP3 of adeno-associated virus (AAV) and at a reduced degree with assembled viral particles. VP1 and VP2 are highly enriched in the nucleus, while non-assembled VP3 is evenly distributed in the nucleus and the cytoplasm. Epitope mapping experiments (Wobus et al., 2000) identified aa726 to aa733 (C-terminus; common to all 3 VP proteins) as the specific binding region. The antibody is also useful for characterization of different stages of infection.
  The AFDye™ 488 is a green emitting dye, structurally and spectrally equivalent to Alexa Fluor® 488 and therefore ideally suited for the 488 nm laser line (excitation/emission 494/517 nm). It can be used with a common FITC filter set.
  Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.
  Wobus, C. E. et al. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74, 9281–93 (2000).
  
  More Details
  
  AAV Antibodies
Properties
- Host
  
  mouse
  
  Antibody Type
  
  monoclonal
  
  Isotype
  
  IgG1
  
  Reactivity
  
  virus (AAV1, AAV2, AAV3, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh10, AAVDJ)
  
  Clone
  
  B1
  
  Immunogen
  
  AAV2 capsids
  
  Purification
  
  protein A affinity chromatography
  
  Conjugate
  
  Alexa-fluor®-488
  
  Formulation
  
  liquid; PBS + 0.09% sodium azide
  
  Storage
  
  up to 1 month: 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles; store in the dark.
  
  * Research use only