Mitochondrial Tracker (Red)

Mitochondrial Tracker (Red) is a red fluorescent dye containing a chloromethyl functional group,which can be used to label mitochondria. Mitochondrial Tracker (Red) can passively diffuse across the cell membrane and directly accumulate in active mitochondria simply by incubation with live cells. The accumulation of this dye depends on the membrane potential. Once the mitochondria are stained, they can be fixed with aldehyde fixatives according to the requirements of subsequent experiments. For experiments such as immunohistochemistry and in situ hybridization, cells need to be permeabilized first, and Mitochondrial Tracker (Red) can also stain the mitochondria of permeabilized cells. This dye is suitable for double-labeling experiments, as its red fluorescence can be well distinguished from other green fluorescent probes. Although traditional mitochondrial fluorescent probes such as TMR and rhodamine 123 can also easily accumulate in functional mitochondria, they will be washed away once the mitochondrial membrane potential is lost. This limits their application in experiments that require aldehyde fixation of cells or involve factors affecting mitochondrial energy status.

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