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Overview
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JC-1 is an ideal fluorescent probe widely used to detect mitochondrial membrane potential. It can be used to detect the mitochondrial membrane potential of cells, tissues or purified mitochondria. When the mitochondrial membrane potential is low, JC-1 cannot aggregate in the mitochondrial matrix. At this time, JC-1 is a monomer with a maximum emission wavelength of 527 nm, which can produce green fluorescence; when the mitochondrial membrane potential is high, JC-1 aggregates in the mitochondrial matrix to form polymers (J-aggregates) with a maximum emission wavelength of 590 nm, which can produce red fluorescence. In this way, it is very convenient to detect changes in mitochondrial membrane potential by changing the fluorescence color.
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- Properties
- Applications
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Overview