Microbial transglutaminase, production grade, characterized for endotoxin, HCP, DNA, sterility

- Overview
  - Please contact us at for specific academic pricing.
    
    Background
    
    Microbial Transglutaminase (MTG) catalyzes the acyl transfer reaction between γ-carboxyamide groups of peptide bound glutamine residues and a variety or primary amines.MTG can be used to covalently cross-link proteins with each other. Here, the ε-amino group of protein bound lysine serves as primary amine. However, MTG also accepts non-protein primary amines as substrate, e.g. amino-butane or amino-pentane. Therefore, MTG can be used to attach primary-amine coupled functional markers like biotin, fluorescent dyes, click chemistry reagents or cytotoxins to proteins, e.g. antibodies.Essentially, MTG is used for the production of antibody drug conjugates (ADCs).
    
    More Details
    
    Zedira
- Properties
  - Name
    
    EC 2.3.2.13; MTG, BTG, BTGase, Protein-glutamine-γ-glutamyltransferase
    
    Source
    
    E. coli
    
    Type
    
    Recombinant Proteins
    
    Formulation
    
    The purified transglutaminase is lyophilized from a solution in the following buffer: 50 mM NaOAc pH 5.0 + 0.3 M NaCl.
    
    Storage
    
    Store working aliquots at ≤ - 20°C. Avoid repeated freezing and thawing.
    
    Note
    
    INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.
    
    Molecular Weight
    
    38,333 Da (Dispase-activated MTG with N-terminal sequence: FRAPDSDDR...)
    
    Class
    
    Microbial (Pro)TGase
    
    Reconstitution
    
    Add the volume of H2O the protein is lyophilized from (see Certificate of Analysis) to the vial of lyophilized powder. Rotate vial gently until solid dissolves.Reconstituted MTG solution might be turbid. However, MTG activity is not affected. In this case, centrifuge the solution and collect the supernatant.After reconstitution, the solution should be stored frozen in working aliquots.
    
    More Information
    
    Animal-Free
    
    yes
- Applications
  - Application Description
    
    Labeling, immobilisation, conjugation and modification of proteins.MTG can tolerate up to 10% DMSO in aqueous solutions without significant influence on its catalytic activity. A small activity loss of 4% was observed in the presence of 20% DMSO.
- Reference
  - Grossowicz et al., J. Biol. Chem. 1950, 187, 111-25
    Ando et al., Agric. Biol. Chem. 1989, 53, 2613-17
    Pasternack et al., Eur. J. Biochem. 1998, 257, 570-6
    Pfleiderer et al., Microbiol. Res. 2005, 160, 265-71
    Jeger et al., Angew. Chem. Int. Ed. Engl. 2010, 49, 9995-7
    Kaufmann et al., Food Addit. Contam Part. A 2012, 29, 1364-73
    Dennler et al., Bioconjugate Chem. 2014, 25, 569-78