Microbial Transglutaminase (MTG) ELISA

Microbial Transglutaminase (MTG) ELISA

Catalog Number:
EK01055388ZED
Mfr. No.:
E021
Price:
$817
  • Size:
    1 Kit
    Quantity:
    Add to Cart:
      • Overview
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          Background

          Microbial transglutaminase catalyzes acyl transfer reactions between the γ-carboxyamide group of a protein-bound glutamine residue and primary amines. In simple terms, microbial transglutaminase acts as a biological glue that covalently links proteins. Among others microbial transglutaminase is used in the food industry for the production of dairy products, meat, sausages and bakery products.
          The Microbial Transglutaminase (MTG) ELISA (Product Nr. E021) is a sandwich enzyme immunoassay developed for the quantitative determination of native MTG. As part of the kit development, the following foods were examined: glued meat (raw), yoghurt, quark, cheese, salami and bread. It can be assumed that the test is also suitable for the analysis of further foods. Detection and determination limits depend on the respective sample matrix, the degree of processing and the extraction process.
          Please note that for each type of sample material, the preparation procedure must be validated separately by the user.
          All reagents for the implementation of the enzyme immunoassay are contained in the test kit. A test kit is sufficient for 96 determinations (including standards). A microtiter plate photometer is required for evaluation.
          Sample preparation: homogenizing, extracting
          Standard material: Microbial Transglutaminase (0 - 150 ng/mL)
          Detection limit: 0.6 ng/mL (ppb) MTG (1 mL ≙ 1 g)
          Determination limit: 3.0 ng/mL (ppb) MTG (1 mL ≙ 1 g)

          More Details

      • Properties
        • Formulation
          Microtiter plate packed in a resealable foil laminate pouch with a dessicant bagSample bufferWash bufferMTG-standard.Purified anti-microbial transglutaminase antibody labeled with biotin (MTG-Ab conjugate)Streptavidin-HRP conjugateSubstrate solutionStop solution (0.2 M H2SO4)Directions for use
          Storage
          Store the whole kit at 6 - 8°C. It is important that the MTG-standard is not stored below 6°C. The kit is stable up to the expiry date stated on the label of the box. Do not use kit beyond its expiry date. After opening the pouch keep unused microtiter wells resealed to minimize exposure to moisture. Substrate solution is sensitive to light, therefore avoid direct exposure to light. After the expiry date (see kit label) no quality guarantee is given.

          * INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

      • Applications
        • Application
          ELISA

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