METAFECTENE® EASY Kit - DNA Transfection Reagent

- Overview
  - METAFECTENE® EASY Kit using a fixed DNA-lipid ratio, rendering time-consuming optimization obsolete, enables high efficient DNA transfection. This reagent shows no serum inhibition and low toxicity, thus it is the reagent of choice for sensitive cells.
    
    Highlights
    ·Suitable for DNA transfection
    ·Easy: Optimization involves only two fixed DNA:lipid ratios
    ·Fast: Results within 2-3 days → two interdependent assays within a week
    ·Efficient for a majority of cell types
    
    Sizes
    2 ml (10×) Buffer: EASY Buffer(10×) 2 ml
    1 × 1 ml Kit: 1 ml METAFECTENE® EASY Transfection reagent with 2 ml EASY Buffer (10×)
    2 × 1 ml Kit: 2 × 1 ml METAFECTENE® EASY Transfection reagent with 2 × 2 ml EASY Buffer (10×)
    5 × 1 ml Kit: 5 × 1 ml METAFECTENE® EASY Transfection reagent with 5 × 2 ml EASY Buffer (10×)
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    Mammalian Cell Line Transfection Reagent
    
    Other Properties
    
    Shelf Life: 1 year (from date of delivery)
    Assays: Up to 750 (24 well) or up to 160 (6 well) per 1 ml reagent
    
    Storage
    
    4℃
    
    Shipping
    
    At room temperature
    
    * Only for research purposes in vitro, not intended for human or animal diagnostic, therapeutic or other clinical uses.
- Applications
  - Application
    
    Mammalian Cell Line Transfection
    
    Application Description
    
    Suitable for a wide range of mammalian cell lines and primary cells.
    Significantly improves transfection efficiency and minimizes optimization efforts.
- Reference
  - "Structural, biocomplexation and gene delivery properties of hydroxyethylated gemini surfactants with varied spacer length." Zakharova et al., 2016, Colloids and Surfaces B: Biointerfaces, 140: 269-277
    "CDKL5 deficiency predisposes neurons to cell death through the deregulation of SMAD3 signaling." Fuchs et al., 2019, Brain Pathology, 29.5: 658-674
    "OCT4 silencing triggers its epigenetic repression and impairs the osteogenic and adipogenic differentiation of mesenchymal stromal cells." Malvicini et al., 2019, International journal of molecular sciences, 20.13: 3268

MSDS

Documents

Product Information and Protocol

Seeding cells and transfection can be done quickly with the METAFECTENE® EASY Kit. A time saving of 24 hours can be achieved, allowing two successive result-dependent transfections to be performed within a single working week.

Recommended applications, sorted by nucleic acid

METAFECTENE® EASY Kit - DNA Transfection Reagent

METAFECTENE® EASY Kit - DNA Transfection Reagent

Documents

Seeding cells and transfection can be done quickly with the METAFECTENE® EASY Kit. A time saving of 24 hours can be achieved, allowing two successive result-dependent transfections to be performed within a single working week.

Recommended applications, sorted by nucleic acid

METAFECTENE® EASY Kit - DNA Transfection Reagent

METAFECTENE® EASY Kit - DNA Transfection Reagent

Documents

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