Medium Binding 96 well plates - 8 Well Breakable Strips on single well holding frame

Medium Binding 96-Well Breakable Strip Plates: Composed of 12 breakable strips of 8 wells, these plates allow easy customization by breaking and repositioning strips or single wells as required.

Medium Binding 96-Well Plates are designed for immunoassays where passive adsorption of large molecules is critical. With a hydrophobic surface that accommodates molecules with hydrophobic regions, they offer a binding capacity of 100 to 200 ng IgG/cm², making them ideal for assays like ELISA, Chemiluminescence (CLIA), Luminescence (LIA), and Fluorescence (FIA).
Medium Binding 96-Well Plates are available in clear, white, and black polystyrene in Breakable Strip, Strip and Solid format. They are suitable for ELISA, Luminescence, Fluorescence, and Chemiluminescent assays.

Surface:
The hydrophobic surface on Medium Binding 96-Well Plates is optimized for passive adsorption of large molecules, including antibodies with hydrophobic regions. This surface delivers a binding capacity of approximately 100 to 200 ng IgG/cm², ensuring effective immobilization of proteins for sensitive detection.

Hydrophobic Medium Binding Surface: Supports the passive adsorption of large molecules, particularly hydrophobic proteins (>10 kDa).
· Binding Capacity: Approximately 100 to 200 ng IgG/cm²
· Low Fluorescence Polystyrene: Manufactured with pure high-quality polystyrene, minimizing background noise and improving optical clarity.
· Wells design: flat bottom.
· Multiple Formats: Available in breakable strip plates, non-breakable strip plates, and solid plates for flexibility in applications.
· Colors: Available in clear, white, and black. Customizable upper rim colors upon request.
Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays.
Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
· Automation-Compatible: Easily integrated with high-throughput automated systems for liquid handling, washing, and analysis.
· Alphanumeric Coding: For easy and accurate well identification during experiments.
· Recommended working volume: of 75 to 300 μl
· Unit: 25 plates
· Minimum order: 50 platesReady to use

Key Benefits:
· Plates Design:
The mould design provides optical quality, important to reduce the background signal.
The rim protects the external face of the bottom from scratches.
· Improved Washing Efficiency: Radius-edged well bottoms enhance washing, reducing residual materials and improving assay consistency.
· Reduced Cross-Contamination: Designed to minimize the risk of cross-contamination between wells, ensuring precise sample isolation.
· Enhanced Imaging: The flat surface provides a clear focal plane, essential for high-quality imaging in microscopy, especially for fluorescence and phase-contrast analysis.
· Improved Mixing: The flat-bottom design facilitates better sample mixing, ensuring consistent results across assays.

Please contact us at for specific academic pricing.

Background

Medium binding capacity
Medium Binding Capacity Surface is suitable for passive adsorption of proteins with different grades of hydrophobicity, especially for those molecules that have a large hydrophobic region.
Medium Binding Capacity surface is an hydrophobic surface suitable for passive adsorption of proteins with different grades of hydrophobicity;
Medium Binding Capacity surface is used in assays in which the adsorbed molecule presents large hydrophobic regions, such as antibodies. This surface is ideal for immunoassays with a binding capacity of 100 to 200 ng IgG/cm2.
Furthermore, this surface is highly selective and shows high affinity towards hydrophobic polypeptides that present a molecular weight > 10 kDa.

Biomat Medium Binding Capacity Performance
Read this technical note: Biomat has carried out a test for checking the properties of its Medium Binding Capacity Surface when IgG were coated on surface.

· Medium Binding Capacity Surfaces main features
Biomat Medium Binding Capacity products have the following characteristics:
Ready to Use
Manufactured under ISO:9001 guidelines
All lots are tested for uniformity and reproducibility
Certificate of Quality is released for every lot
For Research Use Only

· Also, Medium Binding Capacity Products ensures:

Uniformity
Microplates show a CV% less than 5 using:

Human IgG as a coating molecule in an ELISA format, anti Human IgG-HRP as detector and TMB as substrate
Biotinylated albumin as a coating molecule in an ELISA format, streptavidin-HRP as detector and TMB as substrate
Storage
The microplates are stored at room temperature in sealed PE bags.