High Binding 96 well plates - 8 Well Breakable Strips on single well holding frame

High Binding 96-Well Breakable Strip Plates: Composed of 12 breakable strips of 8 wells, these plates allow easy customization by breaking and repositioning strips or single wells as required.

Optimized for high-sensitivity immunoassays, High Binding 96-Well Plates provide reliable performance across various diagnostic and research applications. These plates feature a hydrophilic surface designed for passive adsorption of proteins, offering a binding capacity of 400 to 500 ng IgG/cm².

High Binding 96-Well Plates are available in clear, white, and black polystyrene in Breakable Strip, Strip and Solid format. They are suitable for ELISA, Luminescence, Fluorescence, and Chemiluminescent assays.

Surface:
The hydrophilic surface on High Binding 96-Well Plates is designed for optimal protein adsorption, enhancing the performance of various immunoassays:
High Binding Capacity: Capable of binding 400-500 ng IgG/cm², ensuring effective immobilization of proteins for sensitive detection.Selective Adsorption: Demonstrates strong affinity even at very low protein concentrations (< 50 ng/cm²), enabling enhanced sensitivity and reliable results, especially in low-volume assays.

Key features of High Binding 96-Well Plates
· Hydrophilic High Binding Surface: Allows passive adsorption of proteins with varying hydrophilicity, and is highly selective even at low protein concentrations (< 50 ng/cm²).
· Binding Capacity: Approximately 400 to 500 ng IgG/cm2.
· Low Fluorescence Polystyrene: Manufactured with pure high-quality polystyrene, minimizing background noise and improving optical clarity.
· Wells design: flat bottom.
· Multiple Formats: Available in breakable strip plates, non-breakable strip plates, and solid plates for flexibility in applications.
· Colors: Available in clear, white, and black polystyrene.
· Customizable upper rim colors upon request.
Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays.
Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
· Automation-Compatible: Easily integrated with high-throughput automated systems for liquid handling, washing, and analysis.
· Alphanumeric Coding: For easy and accurate well identification during experiments.
· Recommended working volume: of 75 to 300 μl
· Unit: 50 plates
· Minimum order: 50 plates
· Packaging: 25 per pack
· Ready to use

Key Benefits:
· Plates Design:
The mould design provides optical quality, important to reduce the background signal.
The rim protects the external face of the bottom from scratches.
· Improved Washing Efficiency: Radius-edged well bottoms enhance washing, reducing residual materials and improving assay consistency.
· Reduced Cross-Contamination: Designed to minimize the risk of cross-contamination between wells, ensuring precise sample isolation.
· Enhanced Imaging: The flat surface provides a clear focal plane, essential for high-quality imaging in microscopy, especially for fluorescence and phase-contrast analysis.
· Improved Mixing: The flat-bottom design facilitates better sample mixing, ensuring consistent results across assays.

Please contact us at for specific academic pricing.

Background

High binding capacity
High Binding Capacity Surface is highly selective and shows high affinity towards the adsorption of molecules also when those are present in very small amounts allowing to obtain the maximum sensitivity of the test.
High Binding Capacity surfaces are used in assays where the adsorbed molecule exceeds (up to 400 ng/cm2) the complementary molecules that have to be detected.

Furthermore, this surface is highly selective and shows high affinity towards the adsorption of molecules also when those are present in very small amounts (<50 ng/cm2) allowing to obtain the maximum sensitivity of the test.

· Examples of possible applications for High Binding Capacity surface:
Protein – Antibody Anti-IgM adsorbed onto solid phase used in IgM capture assays
Lipo-protein – Rubella antigen adsorbed onto solid phase used in IgG assays
ELISA (competitive) tests for Steroid Hormones and TSH

· High Binding Capacity Surfaces main features
Biomat High Binding Capacity products have the following characteristics:
Ready to Use
Manufactured under ISO:9001 guidelines
All lots are tested for uniformity and reproducibility
Certificate of Quality is released for every lot
For Research Use Only

· Also, High Binding Capacity Products ensures:

Uniformity
Microplates show a CV% less than 5 using:

Human IgG as a coating molecule in an ELISA format, anti Human IgG-HRP as detector and TMB as substrate
Biotinylated albumin as a coating molecule in an ELISA format, streptavidin-HRP as detector and TMB as substrate
Storage
The microplates are stored at room temperature in sealed PE bags.