Maleimido-C3-NTA - Amerigo Scientific

- Overview
  - Maleimido-C3-NTA is utilized to modify the surfaces on which thiol groups are attached. Through the NTA moiety attached to the surface, genetically expressed protein, bearing a hexahistidine extension at its terminus, can be immobilized via Ni (II) (His-Tag method). Using this technique, Dr. Noji and co-workers were able to directly observe the rotation of F1-ATPase with a fluorescence microscope.
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    Chelate Labeling
    
    Molecular Formula
    
    C18H23N3Na2O9 · H2O
    
    Molecular Weight
    
    489.38
    
    Appearance
    
    White pale yellowish-white powder
    
    Purity
    
    ≥97.0% (HPLC)
    
    Storage
    
    0-5°C
    
    Shipping
    
    ambient temperature
    
    * For research use only
- Reference
  - 1) E. Hochuli, H. Doeli and A. Schacher, New Metal Chelate Adsorbent Selective for Proteins and Peptides Containing Neighbouring Histidine Residues, J. Chromatogr., 1987, 411, 177.
    
    2) E. Hochuli, Large-Scale Chromatography of Recombinant Proteins, J. Chromatogr., 1988, 444, 293.
    
    3) Y. C. Sasaki, Y. Suzuki and T. Ishibashi, Fluorescent X-ray Interference from a Protein Monolayer, Science, 1994, 263, 62.
    
    4) G. B. Sigal, C. Bamdad, A. Barberis, J. Strominger and G. M. Whitesides, A Self-Assembled Monolayer for The Binding and Study of Histidine-Tagged Proteins by Surface Plasmon Resonance, Anal. Chem., 1996, 68, 490.
    
    5) E. L. Schmid, T. A. Keller, Z. Dienes and H. Vogel, Reversible Oriented Surface Immobilization of Functional Proteins on Oxide Surface, Anal. Chem., 1997, 69, 1979.