Maleimide Coated - 96 well Solid plates

Maleimide Plates 96-Well Solid Plates: Solid format designed for high-performance assays.

Maleimide Plates are ideal for assays that require site-directed orientation of specific biomolecules. The surface of these plates provides an effective platform for binding biomolecules with free sulfhydryl groups, such as peptides containing a terminal cysteine or thiol-containing haptens, as well as reducible disulfide bonds. These plates address challenges associated with coating these biomolecules onto standard polystyrene surfaces, offering enhanced binding and assay performance.

Maleimide Coated 96 Well Plates are pre-blocked in order to minimize any non-specific binding and to ensure long-term stability.

Maleimide Coated Plates are available in clear, white, and black polystyrene in Breakable Strip, Strip and Solid format. They are suitable for ELISA, Luminescence, Fluorescence, and Chemiluminescent assays.

Surface:
The Maleimide Coated Surface provides a powerful platform for binding biomolecules containing free sulfhydryl groups, such as peptides with terminal cysteine or thiol-containing haptens, as well as reducible disulfide bonds. These plates are highly effective for assays requiring site-directed orientation, ensuring precise and stable attachment of target molecules for reliable results.

Key features of Malemide Plates
· Maleimide surface: Effective platform for binding biomolecules with free sulfhydryl groups.
· Blocking: Plates are pre blocked with standard proteic blocking (ELISA Blocking) or with non proteic blocking (BlockerWell)
· Low Fluorescence Polystyrene: Manufactured with pure high-quality polystyrene, minimizing background noise and improving optical clarity.
· Wells design: flat bottom.
· Multiple Formats: Available in breakable strip plates, non-breakable strip plates, and solid plates for flexibility in applications.
· Colors: Available in clear, white, and black. Customizable upper rim colors upon request.
Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays.
Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
· Automation-Compatible: Easily integrated with high-throughput automated systems for liquid handling, washing, and analysis.
· Alphanumeric Coding: For easy and accurate well identification during experiments.
· Recommended working volume: of 50 to 100 μl
· Packaging: Each coated plate is packed in a single barrier bag with desiccant
· Unit: Contains 5 plates
· Minimum order: 10 plates.
· Ready to use

Key Benefits
· Plates Design:
The mould design provides optical quality, important to reduce the background signal.
The rim protects the external face of the bottom from scratches.
· Improved Washing Efficiency: Radius-edged well bottoms enhance washing, reducing residual materials and improving assay consistency.
· Reduced Cross-Contamination: Designed to minimize the risk of cross-contamination between wells, ensuring precise sample isolation.
· Enhanced Imaging: The flat surface provides a clear focal plane, essential for high-quality imaging in microscopy, especially for fluorescence and phase-contrast analysis.
· Improved Mixing: The flat-bottom design facilitates better sample mixing, ensuring consistent results across assays.

Please contact us at for specific academic pricing.

Background

Maleimide Coated Surface offers a powerful instrument for binding biomolecules containing free sulfhydryl groups or reducible disulfide bonds that are difficult to coat onto polystyrene plates.
Maleimide Coated Surfaces offer a powerful instrument for binding biomolecules containing free sulfhydryl groups (e.g. peptides that contain a terminal cysteine or thiol containing haptens), or reducible disulfide bonds that are difficult to coat onto polystyrene plates. These coated products are a very useful tool for assays requiring site-directed orientation of particular biomolecules especially during antibody production.

At pH 6.5-7.5 maleimide reacts with free sulfhydryl groups to yield stable bonds, while the reaction with amine becomes significant at pH > 7.5.

If sulfhydryl-containing peptides and proteins oxidize in solution and form disulfide bonds, they must be preventively reduced to free sulfhydryls for allowing interaction with maleimide.

· Maleimide Coated Surfaces main features
Biomat Maleimide Coated products have the following characteristics:

Ready to Use
Manufactured under ISO:9001 guidelines
All lots are tested for uniformity and reproducibility
Certificate of Quality is released for every lot
For Research Use Only
Also, Maleimide Coated Products ensures:

Uniformity
The Maleimide Coated Products show a CV% less than 10 when used as a catcher of Glutathione Ethyl Ester Biotin Amide (BioGEE) in an ELISA format using streptavidin-HRP as detector and TMB as substrate

Storage and Stability
The Maleimide Coated products, if unopened, are stable at 2 – 8°C until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.

· Maleimide Coated Microplates Specifications
Coating
A derived maleimide is coated using 100 μl/well.

Binding capacity
Microplate was saturated with Glutathione Ethyl Ester Biotin Amide (BioGEE) at a concentration of 2.5 µg/ml (250 ng/well) in an ELISA format using Streptavidin-HRP as detector and TMB as substrate.

Maleimide Coated PCR 8 Strip Tubes and PCR Plates Specifications
Coating
A derived maleimide is coated using 100 μl/tube.