Goat anti-Rabbit IgG Coated - 96 well Solid plates

Goat Anti-Rabbit IgG Plates 96-Well Solid Plates: Solid format designed for high-performance assays.

The Goat Anti-Rabbit IgG Plates are designed to specifically bind the Fc region of rabbit immunoglobulins, with minimal cross-reaction to human serum proteins. These plates are ideal for binding assays where antibodies are in low quantities or may denature and lose activity when directly adsorbed onto polystyrene.

The Goat Anti-Rabbit IgG Coated Plates prevent antibody denaturation caused by direct adsorption on polystyrene, ensuring antibodies remain active. They offer high specificity, unlike Protein A or G plates, they provide selective binding specifically to target rabbit IgG. Additionally, these plates deliver enhanced antibody-binding capacity, especially when using diluted rabbit antibody solutions, outperforming direct adsorption to polystyrene.

Secondary Antibodies Goat Anti Rabbit IgG Coated Plates are pre-blocked in order to minimize any non-specific binding and to ensure long-term stability.

Goat Anti-Rabbit IgG Plates are available in clear, white, and black polystyrene in Breakable Strip, Strip and Solid format. They are suitable for ELISA, Luminescence, Fluorescence, and Chemiluminescent assays.

Surface:
The Goat Anti-Rabbit IgG surface is ideal for assays where antibodies are present in low quantities or risk denaturation and inactivation when directly adsorbed to polystyrene plates. These plates specifically bind to the Fc region of rabbit immunoglobulins, with minimal cross-reactivity to human serum proteins, ensuring reliable performance in sensitive assays.

Key features of Goat Anti-Rabbit IgG Plates
· Prevents Antibody Denaturation: Protects antibodies from inactivation due to direct adsorption on polystyrene.
· Selective Binding: Unlike Protein A or G plates, these plates bind specifically to target mouse IgG, ensuring higher specificity.
· Enhanced Binding Capacity: Offers superior antibody-binding capacity compared to direct adsorption on polystyrene, particularly when using diluted rabbit solutions.
· Blocking: Plates are pre blocked with standard proteic blocking (ELISA Blocking) or with non proteic blocking (BlockerWell)
· Low Fluorescence Polystyrene: Manufactured with pure high-quality polystyrene, minimizing background noise and improving optical clarity.
· Wells design: flat bottom.
· Multiple Formats: Available in breakable strip plates, non-breakable strip plates, and solid plates for flexibility in applications.
· Colors: Available in clear, white, and black. Customizable upper rim colors upon request.
Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays.
Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
· Automation-Compatible: Easily integrated with high-throughput automated systems for liquid handling, washing, and analysis.
· Alphanumeric Coding: For easy and accurate well identification during experiments.
· Recommended working volume: of 75 to 200 μl
· Packaging: Each coated plate is packed in a single barrier bag with desiccant
· Unit: Contains 5 plates
· Minimum order: 10 plates.
· Ready to use

Key Benefits
· Plates Design:
The mould design provides optical quality, important to reduce the background signal.
The rim protects the external face of the bottom from scratches.
· Improved Washing Efficiency: Radius-edged well bottoms enhance washing, reducing residual materials and improving assay consistency.
· Reduced Cross-Contamination: Designed to minimize the risk of cross-contamination between wells, ensuring precise sample isolation.
· Enhanced Imaging: The flat surface provides a clear focal plane, essential for high-quality imaging in microscopy, especially for fluorescence and phase-contrast analysis.
· Improved Mixing: The flat-bottom design facilitates better sample mixing, ensuring consistent results across assays.

Please contact us at for specific academic pricing.

Background

Biomat's Secondary Antibodies Coated Surface, available with Goat Anti-Mouse IgG and Goat Anti-Rabbit IgG, is specifically designed for antibody-binding assays where direct adsorption to polystyrene can compromise antibody integrity. This surface is ideal for cases where primary antibodies are limited in quantity or prone to denaturation, ensuring optimal assay sensitivity and consistency.

· When and Why to Use a Secondary Antibody Coated Surface
Prevents antibody denaturation caused by direct adsorption onto polystyrene
Ensures higher binding efficiency for diluted antibody solutions, especially mouse and rabbit IgG
Fc-specific binding offers greater selectivity than Protein A or Protein G plates
Ideal for indirect ELISA, sandwich ELISA, competitive assays, isotyping, and hybridoma screening

Unlike Protein A or G-coated surfaces, Biomat's secondary antibody-coated surfaces bind exclusively to the IgG class of the selected species, offering enhanced specificity and compatibility with downstream detection protocols.

· Key Features of Biomat's Secondary Antibody Coated Surface
Biomat Coated Plates with Secondary Antibodies are suitable with ELISA Assay Technology due to these features:
Fc-specific surface binding for mouse and rabbit IgG
Ready-to-use 96-well format
Made of pure polystyrene with low fluorescence background
Post-coated (blocked) to minimize non-specific binding
Alphanumeric coding for accurate well identification
SBS-standard compliant, automation-friendly design
Manufactured under ISO 9001:2015 quality standards
Lot-specific Certificate of Quality included
For Research Use Only

· Surface Performance & Uniformity
Uniform coating ensures coefficient of variation (CV%) below 5%

Compatible with ELISA workflows using anti-IgG-HRP conjugates and TMB substrate

Reliable detection of low-abundance antibodies across multiple applications

· Storage and Stability
Store unopened plates at 2–8°C until expiration date (printed on the label)

After opening, keep in sealed pouch with desiccant; use before expiration

Secondary Antibodies Coated Surfaces Product specifications

· Goat Anti Mouse IgG Fcγ (Subclasses 1+2a+2b+3)
Affinity purified goat anti mouse IgG specifically binds the Fc region of mouse immunoglobulin subclasses 1, 2a, 2b and 3, with minimal cross-reaction to human, bovine and rabbit serum proteins.

These plates may be used as solid support for most sandwich ELISAs utilizing a mouse IgG capture and a non mouse IgG detection antibody. Other applications include competitive ELISA, IgG isotyping and hybridoma screening/selection.

Examples of applications for Goat Anti Mouse IgG Fcγ Coated 96-Well Plates:

prevent antibody denaturation as a result of direct adsorption to polystyrene
unlike Protein A or G plates, these plates bind only to target IgG species
these plates show a higher antibody-binding capacity than direct adsorption onto polystyrene when using diluted mouse IgG solutions

· Goat Anti Mouse IgG Fcγ Coated 96-Well Plates Product Specifications
Coating
Affinity purified goat anti mouse IgG Fcγ (subclasses 1+2a+2b+3) is coated using 100 μl/well.

Binding capacity
Microplate was saturated with mouse IgG at a concentration of 1.0 μg/ml (100 ng/well) in an ELISA format using goat anti mouse IgG (H+L)-HRP as detector and TMB as substrate.

Sensitivity
Mouse IgG was detected at a concentration significantly above background in an ELISA format using goat anti mouse IgG (H+L)-HRP as detector and TMB as substrate.

· Goat Anti Rabbit IgG Fc
Affinity purified goat anti rabbit IgG specifically binds the Fc region of rabbit immunoglobulins, with minimal crossreaction to human serum proteins.

These plates may be used as solid support for most sandwich ELISA utilizing a mouse IgG capture and a non mouse IgG detection antibody. Other applications include competitive ELISA, IgG isotyping and hybridoma screening/selection.

Examples of applications for Goat Anti Rabbit IgG Fc Coated 96-Well Plates:

prevent antibody denaturation as a result of direct adsorption to polystyrene
unlike Protein A or G plates, these plates bind only to target IgG species
these plates show a higher antibody-binding capacity than direct adsorption onto polystyrene when using diluted rabbit solutions
Goat Anti Rabbit IgG Fc Coated 96-Well Plates Product Specifications
Coating
Affinity purified Goat anti rabbit IgG Fc is coated using 100 μl/well.

Binding capacity
Microplate was saturated with rabbit IgG at a concentration of 1.0 μg/ml (100 ng/well) in an ELISA format using goat anti rabbit IgG (H+L)-HRP as detector and TMB as substrate.

Sensitivity
Rabbit IgG was detected at a concentration significantly above background in an ELISA format using goat anti rabbit IgG (H+L)-HRP as detector and TMB as substrate.