MAL-dPEG®₈-acid

MAL-dPEG®8-acid, product number 10275, is a crosslinker that joins sulfhydryl groups to free amines through a single molecular weight polyethylene glycol (PEG) chain of discrete length. The maleimidopropyl moiety reacts with sulfhydryl groups via a Michael addition reaction. The propionic acid terminus of the molecule reacts directly with free amines using EDC or another carbodiimide to form amide bonds. Alternatively, activation of the propionic acid group with N-hydroxysuccinimide (NHS), 2,3,5,6-tetrafluorophenol (TFP), or some other acylating agent permits amide bond formation under mild, controlled conditions.

Crosslinking with dPEG® Products
Chemical couplings between free amines and free thiols are among the most popular, most useful crosslinking reactions in bioconjugate chemistry. These syntheses require heterobifunctional reagents that bridge the two groups.

Traditional crosslinkers are hydrophobic molecules. However, our crosslinking products are water-soluble, amphiphilic, single molecular weight PEG compounds with discrete chain lengths, hence the dPEG® tradename under which we sell them.

Almost inevitably, the conjugation of conventional hydrophobic crosslinking reagents to biomolecules triggers problems such as aggregation and precipitation of the conjugates. Conversely, these problems do not occur with our water-soluble, non-immunogenic dPEG® crosslinkers.

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