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Overview
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This product supplies a simple and rapid extraction of total RNA from plant and fungal samples. The kit is based on super paramagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction process takes only 60 minutes. Purified RNA is ready for downstream applications such as RT-PCR, virus RNA testing and so on. MagPure RNA Kits buffers can be used for both manual extraction process and automatic nucleic acid extraction machines. This Kits is suitable for extracting RNA from ≤5x106 cultured cells, 20mg tissue and <50mg plant samples.
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally RNA was eluted by Elution Buffer.
Advantages
• High quality - high purity total RNA can be directly used in various sensitive downstream applications
• Safe - no phenol chloroform extraction required
• Fast - several samples can be extracted in 60 minutes by column method
• Universal - two lysates suitable for most plant or fungal tissue samplesPlease contact us at for specific academic pricing.
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- Properties
- Applications
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Overview