HiPure Circulating Nucleic acid Mini Kit

Free-circulating nucleic acids, such as tumor-specific extracellular DNA fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasma usually as short fragments, <1000bp (DNA). HiPure Circulating DNA Mini Kit enables efficient purification of these circulating nucleic acids from human plasma, serum, or urine. Samples can be fresh or frozen (provided that they have not been frozen and thawed more than once).

Principle
This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption plate and filter column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10 Mm Tris, pH 8.0).

Advantages
• High yield - most optimal process, free DNA (>50bp) can be obtained to the maximum extent
• High concentration - low elution volume, ensuring high nucleic acid concentration
• High purity - low alcohol binding method, completely removing inhibitor and protein pollution
• High recovery - DNA can be recovered at the level of PG by silica gel column purification

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