HiPure Universal DNA Kit

HiPure Universal DNA Kit

Catalog Number:
NAPK357120ANG
Mfr. No.:
D301802; D301803
Price:
$249
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      • Overview
        • This product is suitable for rapid extraction of total DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.

          Principle
          This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

          Advantages
          • High quality DNA - meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
          • Fast - without separation of leukocytes, organic extraction or ethanol precipitation
          • Simple - all nucleic acids can be obtained by direct digestion
          • Wide applicability- handle a variety of liquid samples

          Please contact us at for specific academic pricing.

      • Properties
        • Details
          Purification method: Mini spin column
          Purification technology: Silica technology
          Process method: Manual (centrifugation or vacuum)
          Sample type: Tissue, cell, blood, saliva, swab, blood spot, semen and other clinical samples
          Sample amount: Solid tissue: 1-10mg; Anticoagulant blood: 200μl
          Storage
          Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

          * For Research Use Only

      • Applications
        • Application
          PCR, qPCR, southern bolt and virus detection, etc
          Application Description
          Isolation total DNA from tissue / blood / body fluid / swab /dry blood spots

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