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Overview
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LZCap™AG(3'Ma-Cy7) is a Cap1 analog with a Cy7 fluorescent marker, which can be used as an mRNA co-transcription capping reagent. Under the action of T7 polymerase, LZCap®AG(3'Ma-Cy7), NTPs and template DNA are co-transcribed to produce mRNA with a 5'-end Cap 1 structure. The capped mRNA can be directly translated and expressed in cells and in vivo, with excellent expression efficiency. The mRNA capped by LZCap®AG(3'Ma-Cy7) can be detected by flow cytometry and fluorescence microscopy for Cy7 fluorescence, and the distribution of mRNA and LNP can be traced and located. The recommended wavelength of Cy7 fluorescence of LZCap®AG(3'Ma-Cy7) mRNA is: (651/780).
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Overview