mRNA Vaccinia Capping Enzyme (10 U/μL)

Eukaryotes mRNA forms a special structure at the 5'end after transcription, which is the cap structure. The cap structure plays an important role in the stability, transportation, and translation of mRNA. The vaccinia virus capping enzyme is an effective enzyme that can catalyze the formation of the cap structure. It"s composed of two subunits D1 and D12. It also has RNA triphosphatase activity, guanylate acyltransferase activity, and guanine methyltransferase activity that could connect the 7-methylguanine cap structure (m7Gppp) to the 5'end of the RNA (m7Gppp5'N). Vaccinia virus capping enzyme can cap the RNA in correct direction within one hour when present at a suitable concentration of capping buffer, guanosine triphosphate (GTP), S-adenosylmethionine (SAM), etc.
Feature
• Isolated from a recombinant source
• Tested for the absence of endonucleases, exonucleases, RNases

Application
• Capping mRNA before translation assays/in vitro translation
• Labeling 5´ end of mRNA

Please contact us at for specific academic pricing.