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Overview
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Kit Components: Lentiviral particles or plasmids for a set of three sgRNAs, and a pair of Indel screening PCR primers
sgRNA lentiviral particle information
• Functional titer: 1x10^8 TU/ml
• Titration method: counting puromycin resistant cell clones
• Purity: In vivo grade
• Pseudotype: VSVG
• Storage buffer: LentiShield™ lentivirus storage buffer
• Storage: condition -80°C
• Availability: 3-5 weeks
sgRNA lentiviral plasmid information
• Promoter for target sgRNA: U6
• Mammalian selection: puromycin
• Promoter for mammalian selection gene: EF1a
• E.coli selection: Amp
• Storage condition: -20°C
• Availability: 1-2 weeksPlease contact us at for specific academic pricing.
Target Information
Target NameHuman RASGRP3About TargetGene symbol: RASGRP3
Synonyms: GRP3
Species: HUMAN
Gene ID: 25780
Summary: The protein encoded by this gene is a guanine nucleotide exchange factor that activates the oncogenes HRAS and RAP1A. Defects in this gene have been associated with systemic lupus erythematosus and several cancers. [provided by RefSeq, Mar 2017].
NM numbers targeted (Accession number:Exon:CDS Fragment): NM_001139488:10:8 NM_170672:11:8 NM_015376:9:8 NM_001349975:12:8 NM_001349976:10:8 NM_001349977:11:8 NM_001349978:11:8 NM_001349979:10:8 NM_001349980:11:8 NM_001349981:10:8
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