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Overview
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Lb5Cas12a (previously known as Lb5Cpf1), a DNA endonuclease mediated by crRNA, is derived from Lachnospiraceae bacterium MA2020 strain. Lb5Cas12a can specifically cleave the dsDNA target in a PAM (TTN)-dependent manner, generating DSBs, and cleave ssDNA target in a PAM-independent manner. In addition, both dsDNA and ssDNA targets can trigger the trans-cleavage activity of Lb5Cas12a, that is, when Cas12a binds crRNA and target DNA to form a ternary complex, the trans-cleavage activity of Cas12a will be released to non-specifically cleave ssDNA sequences in the reaction system. Therefore, Lb5Cas12a can be used not only for specific cleavage of dsDNA in vitro, but also for rapid detection of target nucleic acid.(for details, please refer to PMID: 29707234).
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