L-Rhamnose Assay Kit

- Overview
  - The L-Rhamnose Assay Kit for the measurement of L-rhamnose in plant extracts, culture media/supernatants and other materials is a simple, rapid method.
    
    L-Rhamnose occurs naturally in the L-form and is commonly present as a component of the carbohydrate moiety of eukaryotic glycoproteins and in plant cell wall polysaccharides. The most abundant occurrence of L-rhamnose is within the pectic fraction of plant cell wall polysaccharides. L-Rhamnose is commonly used as a non-metabolisable marker along with lactulose for dual-permeability testing in the diagnosis of intestinal diseases such as Crohn’s disease or coeliac disease.
    
    Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
    
    Please contact us at for specific academic pricing.
- Properties
  - Details
    
    Limit of Detection: ~1.2mg/L
    
    Storage
    
    Short term stability: 2-8°C
    Long term stability: See individual component labels
    
    Stability
    
    > 1 year under recommended storage conditions
    
    * For research use only.
- Applications
  - Application Description
    
    Hydrolysates of plant material and polysaccharides, culture media/supernatants and other materials.
- Reference
  - 1.Measurement of available carbohydrates in cereal and cereal products, dairy products, vegetables, fruit and related food products and animal feeds: First Action 2020.07. McCleary, B. V. & McLoughlin, C. (2021). Journal of AOAC International, qsab019.
    2.Stimuli-responsive vesicles as distributed artificial organelles for bacterial activation. Gispert, I., Hindley, J. W., Pilkington, C. P., Shree, H., Barter, L. M., Ces, O. & Elani, Y. (2022). Proceedings of the National Academy of Sciences, 119(42), e2206563119.
    3.Utilization of stalk waste separated during processing of sun-dried figs (Ficus carica) as a source of pectin: Extraction and determination of molecular and functional properties. Çavdaroğlu, E. & Yemenicioğlu, A. (2021). LWT, 154, 112624.
    4.Ability of yeast metabolic activity to reduce sugars and stabilize betalains in red beet juice. Dygas, D., Nowak, S., Olszewska, J., Szymańska, M., Mroczyńska-Florczak, M., Berłowska, J., Dziugan, P. & Kręgiel, D. (2021). Fermentation, 7(3), 105.
    5.A new, quick, and simple protocol to evaluate microalgae polysaccharide composition. Decamp, A., Michelo, O., Rabbat, C., Laroche, C., Grizeau, D., Pruvost, J. & Gonçalves, O. (2021). Marine Drugs, 19(2), 101.
    6.Addition of pectin-alginate to a carbohydrate beverage does not maintain gastrointestinal barrier function during exercise in hot-humid conditions better than carbohydrate ingestion alone. Flood, T. R., Montanari, S., Wicks, M., Blanchard, J., Sharpe, H., Taylor, L., Kuennen, M. R. & Lee, B. J. (2020). Applied Physiology, Nutrition, and Metabolism, 45(10).
    7.Cross-linking of diluted alkali-soluble pectin from apple (Malus domestica fruit) in different acid-base conditions. Gawkowska, D., Cieśla, J., Zdunek, A. & Cybulska, J. (2019). Food Hydrocolloids, 92, 285-292.
    8.Systemic Concocting of Cross‐Linked Enzyme Aggregates of Candida antarctica Lipase B (Novozyme 435) for the Biomanufacturing of Rhamnolipids. Rathankumar, A. K., SaiLavanyaa, S., Saikia, K., Gururajan, A., Sivanesan, S., Gosselin, M., Vaidyanathan, V. K. & Cabana, H. (2019). Journal of Surfactants and Detergents, 22(3), 477-490.
    9.Interactions of anthocyanins with pectin and pectin fragments in model solutions. Larsen, L. R., Buerschaper, J., Schieber, A. & Weber, F. (2019). Journal of Agricultural and Food Chemistry, 67(33), 9344-9353.
    10.Unusual active site location and catalytic apparatus in a glycoside hydrolase family. Munoz-Munoz, J., Cartmell, A., Terrapon, N., Henrissat, B. & Gilbert, H. J. (2017). Proceedings of the National Academy of Sciences, 114(19), 4936-4941.
    11.The effects of acute oral glutamine supplementation on exercise-induced gastrointestinal permeability and heat shock protein expression in peripheral blood mononuclear cells. Zuhl, M., Dokladny, K., Mermier, C., Schneider, S., Salgado, R. & Moseley, P. (2015). Cell Stress and Chaperones, 20(1), 85-93.
    12.Effects of oral glutamine supplementation on exercise-induced gastrointestinal permeability and tight junction protein expression. Zuhl, M. N., Lanphere, K. R., Kravitz, L., Mermier, C. M., Schneider, S., Dokladny, K. & Moseley, P. L. (2014). Journal of Applied Physiology, 116(2), 183-191.

MSDS

L-Rhamnose Assay Kit

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