-
-
Overview
-
Please contact us at for specific academic pricing.
Background
L-690,330 is a competitive inhibitor of inositol monophosphatase (IMPase) with a Ki value of between 0.2 and 2 μM depending on the source of IMPase [1].In the phosphatidylinositol (PI) cycle cell signalling pathway, IMPase is involved as a key enzyme. Inositol monophosphates, inositol(1)phosphate [Ins(l)P], inositol(3)phosphate [Ins(3)P], and inositol(4)phosphate [Ins(4)P] are hydrolyzed by IMPase and produce free inositol. Free inositol can then be used to synthesize PI [1].In carbachol-stimulated m1 CHO cells, the treatment with L-690,330 alone did not increase the amount of [3H]InsP1. In the absence of L-690,330, the treatment with carbachol alone increased levels of [3H]InsP1, resulted in levels ranging from 20 to 100% above basal [3H]InsP1 levels. In the presence of carbachol, lithium at 10 mM increased the amount of [3H]InsP1 by approximate five folds (relative to [3H]InsP1 levels in carbachol-stimulated cells) and the EC50 value of this effect was about 1 mM. In carbachol-stimulated cells, L-690,330 at 10 mM resulted in only ~40% of the maximum response seen in the case with lithium treatment [1]. In mouse brains, in the absence of L-690,330, pilocarpine produced approximately doubled levels of Ins(1)P. In the absence of pilocarpine, L-690,330 did not affect Ins(1)P levels. Under pilocarpine-stimulated conditions, treatment with L-690,330 resulted in a pronounced accumulation of Ins(1)P, with the maximum level observed 1 h after injection. The maximum was of an approximate four-fold elevation [1].
-
- Properties
-
Overview
- Home
- Products
- Life Science
- Analytical Chromatography
- Assay Kits
- Biochemical Reagents
- Antibodies
- Antigens
- Enzymes
- Chemicals
- Proteins
- Liposomes
- Cell Culture
- Cell Analysis
- Cloning and Expression
- Nucleic Acid Synthesis
- Nucleic Acid Extraction
- Nucleic Acid Electrophoresis
- PCR
- Protein Gel Electrophoresis
- Protein Biochemistry
- Nanoparticles
- Glycan Analysis
- Immunohistochemistry (IHC)
- Microbiology
- Biospecimens
- Transfection Reagents
- Ion Exchange Resins
- Venoms
- Lab Solutions
- Lab Equipment
- Biosensors
- Flow Imaging Microscopes
- Fluorescence Quality Management Solutions
- Electrophoresis Systems
- Hematology Analyzers
- Isothermal Amplification Instruments
- Liquid Handling Equipment
- Luminometers
- Microfluidic Flow Control Systems
- Next-generation Sequencing Systems
- Nucleic Acid Purification Instruments
- Raman Spectroscopy Systems
- Sample Tracking Systems
- Spectrometer Accessories
- Syringe Pumps
- Thawing and Warming Systems
- Tissue Dissociation Systems
- Water Purification Systems
- Western Blotting Equipment
- General Laboratory Equipment
- Clinical Chemistry Analyzers
- Amerigo Scientific Instrument
- Automated Cell Selection Systems
- Automated Nucleic Acid Extraction Instruments
- Balances, Scales and Weighing
- Biolayer Interferometry Instruments
- Bioprocess Filtration Equipment
- Bioprocess Systems and Accessories
- Bioreactors and Fermenters
- Cappers and Decappers
- Cell Thawing Instruments
- Chromatography
- Data Loggers
- Droplet Generators
- Dry Baths
- Flow Cytometers
- Histology Laboratory Equipment
- Lab Ovens and Furnaces
- Lab Refrigeration Equipment
- Lab Shakers and Mixers
- Laboratory Centrifuges
- Laboratory Evaporators
- Laboratory Hoods
- Laboratory Incubators
- Laboratory Microscopes
- Laboratory Mills
- Laboratory Pumps
- Liquid Handling
- Live Cell Imaging Systems
- Microbiology Equipment
- Microscopy Flow Cells
- Mutation Breeding Instruments
- Oligonucleotide Synthesis Devices
- Particle Counters
- Plasma Surface Treatment Equipment
- Presses and Pellet Dies
- Surface Plasmon Resonance Instruments
- Ultrasonic Cleaners
- UV Disinfection Systems
- VOC Analysis Equipment
- VPHP Sterilization Systems
- Water Baths
- Lab Equipment
- Diagnostics & Clinical
- Applied Science
- Life Science
- Services
- About Us
- Contact Us
.
