K562-cas9 Cell (Human Chronic Myelogenous Leukemia Cell)

Species: human
Cell Morphology: Suspension cells
Complete Culture Medium Components: 1640+10%FBS
Mycoplasma testing: Negative
Antibiotic Concentration: puromycin, 0.5ug/ml
Cultivation Environment: 37°C, 5% CO₂
Passage Ratio: 1/2 to 1/4 of the generations
Frequency of Reproduction: 2-3 times/week
Gene Expression: Cas9 protein, spCas9, CRISPR-Cas9 protein

Product Advantages:
• Efficiency and Convenience: A "ready-to-use" platform; simply import gRNA, operation is simple, editing efficiency is high, saving time and costs.
• High-Throughput Screening: An ideal foundational platform for genome-wide CRISPR screening, used for systematic discovery of functional genes.
• Consistency and Reproducibility: Consistent cell population genetic background, stable Cas9 expression, more reliable experimental results, and more rigorous control settings.
• Functional Scalability: Cell lines expressing dCas9, high-fidelity Cas9, and other variants can be constructed to achieve multiple functions beyond gene knockout (regulation, imaging, etc.).

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Background

K-562 cells were established by Lozzio from the pleural effusion of a terminally ill 53-year-old female patient with chronic myeloid leukemia. Classified as highly undifferentiated granulocytes, studies by Erson et al. on their surface membrane properties indicate that K-562 cells are a human erythroleukemia cell line. K-562 cells are widely used as highly sensitive in vitro receptors in natural killer (NK) assays. See Pross et al. used K-562 cells for detailed in vitro NK cell assays, including the mathematical quantification of NK cell activity. K-562 blastocytes are pluripotent hematopoietic malignant cells capable of spontaneously differentiating into recognizable erythrocyte progenitors, granulocytes, monocytes, etc. K-562 cells are EBNA negative.