IPTG (Isopropyl β-D-1-thiogalactopyranoside)

- Overview
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    Background
    
    IPTG is an inducer of the lactose system. It induces the lac operon by binding to and releasing the lac repressor allowing transcription of lac genes including beta-galactosidase.
- Properties
  - CAS Number
    
    367-93-1
    
    Molecular Formula
    
    C9H18O5S
    
    Molecular Weight
    
    238.3
    
    Solubility
    
    Soluble in water
    
    Storage
    
    2-8°C
    
    * For research use only
- Applications
  - Application Description
    
    Microbiology Applications: IPTG can be used to confirm successful DNA recombination within the lac operon in cloning experiments. X-gal, or Bluo-Gal chromogenic substrate which turns a blue color when cleaved by IPTG can be used in the media in which the cells are grown. White colonies indicate a gene insertion within the lac operon because beta-galactosidase was not expressed. Blue colonies are indicative of an intact lac operon which expressed beta-galactosidase and subsequently cleaved X-gal.