Inorganic Pyrophosphatase ELISA kit

Inorganic pyrophosphatase (PPase) can catalyze the conversion of one molecule of pyrophosphate into two molecules of phosphate ions. In nucleic acid amplification experiments, PPase can hydrolyze inorganic pyrophosphate generated by the reaction. This is a highly exothermic reaction that can shift the reaction equilibrium toward the product generation end, thereby avoiding inhibition of the reaction system.
In the production process of mRNA vaccine products, the addition of PPase can increase the yield of mRNA. However, for products with mRNA as the final product, the added PPase is an exogenous substance and needs to be removed during the process purification. Therefore, it is necessary to detect the residual amount of PPase to evaluate the removal effect of the purification process on protein residues and to confirm whether the PPase residues in the mRNA vaccine products have been purified to a certain acceptable range.
This kit uses the experimental principle of double antibody sandwich enzyme-linked immunosorbent assay (ELISA) to detect the residual amount of PPase. Add the PPase standard and the sample to be tested to the ELISA plate (36706-A) pre-coated with anti-PPase antibody, then add the diluted biotin-labeled detection antibody (36706-C), and finally add Streptavidin-HRP (SA-HRP) (36706-D) to form an antibody + antigen + antibody-Biotin + SA-HRP complex. After washing the plate, add TMB colorimetric solution (36706-H) for color development. TMB is converted from colorless to blue under the catalysis of HRP enzyme and finally converted to yellow under the action of stop solution (36706-I). The depth of yellow is positively correlated with the amount of PPase detected in the sample.

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