Protein A ELISA Kit

Protein A purification media is widely used in the production of antibody drugs because it has specific adsorption with most antibodies. Protein A is bound to the purification media by covalent bonds, but there is a certain probability that Protein A will fall off and remain during the purification process.
This kit uses the experimental principle of double antibody sandwich enzyme-linked immunosorbent assay (ELISA) to detect the residual amount of Protein A. Protein A standard (36716-B) and the sample to be tested are added to the Protein A capture microwell strip (36716-A) pre-coated with anti-Protein A antibody. Then, the diluted biotin-labeled Protein A detection antibody (36716-C) is added. Finally, Streptavidin-HRP (SA-HRP) (36716-D) is added to form an antibody + antigen + antibody-Biotin + SA-HRP complex. After washing the plate, TMB color development solution (36716-F) is added for color development. TMB is converted from colorless to blue under the catalysis of HRP enzyme and finally to yellow under the action of stop solution (36716-G). The depth of yellow is positively correlated with the amount of Protein A detected in the sample.

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