Inflammation Antibody Array

Inflammation Antibody Array features 94 antibodies for profiling major inflammatory factors including interleukins, chemokines, and other cytokines in human cells, tissues, serum or culture media.

Inflammation Antibody Array is a high-throughput ELISA based antibody array for profiling of major inflammatory factors including cytokines, chemokines and related biomarkers. The array is designed for comparing normal samples to treated or diseased samples, screening and identifying candidate biomarkers.

Key Features:
· 94 inflammation factor antibodies; 6 replicates per antibody
· Qualitative/semi-quantitative protein expression profiling of cytokines and related biomarkers
· Antibodies covalently immobilized on 3D polymer coated glass slide
· Fluorescent detection

Specifications:
· Featured Antibodies: 94 antibodies; 6 replicates per antibody
· Reactivity: Human
· Suitable Sample Type: Cell lysate | Tissue lysate | Serum | Culture Media
· Detection Method: Fluorescence | Compatible Scanners
· Internal Controls: Positive controls: beta-actin | GAPDH | Negative controls
· Product Size: 2 slides (for 2 samples)
· Slide Dimensions: 76 x 25 x 1 mm
Spot diameter: 260 - 280 um

Target Proteins:
Angiopoietin-2, BCA-1/CXCL13, BDNF, CD40L, Receptor, C-reactive, Protein, CXCL16, E-cadherin, EGF, ENA78/CXCL5, Eotaxin/CCL11, Eotaxin-2/CCL24, Eotaxin-3/CCL26, EPCAM, Exodus-2/CCL21, Fas-ligand, Ferritin, FGF-acidic, FGF-basic, Fractalkine/CX3CL1, GCP-2/CXCL6, G-CSF, GDNF, GM-CSF, Granzyme, B, GRO/MGSA/CXCL1, GRO-beta/CXCL2, GRO-gamma/CXCL3, HCC-1/CCL14, Hepatocyte, Growth, Factor, HIF-1a, I-309/CCL1, ICAM-1/CD54, IFN-beta, IFN-gamma, IFN-lamda-2/IL28A, IL-1, alpha, IL-1, beta, IL-10, IL-11, IL-12, IL-13, IL-15, IL-17, (IL-17A), IL-17B, IL-17D, IL-17E, (IL-25), IL-17F, IL-1RA, IL-2, IL-22, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-8/CXCL8, IL-9, IP-10/CXCL10, I-TAC/CXCL11, MCP-1/MCAF/CCL2, MCP-2/CCL8, MCP-3/CCL7, MCP-4/CCL13, M-CSF, MDC/CCL22, Midkine, MIG, /CXCL9, MIP-1alpha/CCL3, MIP-1beta/CCL4, MIP-3/CCL23/CCL6, MIP-3alpha/CCL20, MIP-3beta/CCL19, MIP-4/CCL18, MIP-5/CCL15, Myeloperoxidase, (MPO), PDGF-BB, PF-4/CXCL4, RANTES/CCL5, SDF-1a/CXCL12, TARC, /CCL17, TGF-beta, 1, TGF-a, TGF-b, Receptor, II, TGF-b, Receptor, III, TGF-b1, TGF-b2, TGF-b3, TIMP1, TIMP2, TNF-alpha, TNF-beta, (LTA), VCAM-1, VEGF

Assay Outline:
The ELISA based Explorer Antibody Array platform involves four major steps:
· Protein extraction with non-denaturing lysis buffer
· Biotinylation of protein samples
· Incubation of labeled samples with antibody array
· Detection by dye conjugated streptavidin

Please contact us at for specific academic pricing.

Background

· High-Throughput Antibody Arrays
Our antibody arrays provide a high-throughput, ELISA-based solution for protein expression profiling across samples like cells, tissues, serum, and culture media. It offers greater productivity and cost savings compared to traditional Western Blots or ELISA.

· Comprehensive Protein Profiling
Choose from our comprehensive arrays: Phospho Explorer Array, Signaling Explorer Array, and Explorer Antibody Array. Profile up to 1,358 biomarkers on a single slide, with options tailored to specific pathways in immunology, oncology, and cell biology.

· Phosphorylation Profiling
Our phosphorylation profiling antibody arrays include the Phospho Explorer Array with 1318 antibodies and Pathway Phospho Arrays covering more than 25 different pathways. This platform features phospho-specific antibodies and paired site-specific antibodies for total protein. It is ideal for tyrosine, serine, and threonine phosphorylation analysis.

· High-Quality, Reliable Arrays
Our antibody arrays feature antibodies covalently attached to premium glass with a 3D polymer coating for optimal binding and specificity. Each includes positive/negative controls and multiple replicates for accurate, reliable results.