iClick™ EdU Andy Fluor™ 488 Flow Cytometry Assay Kit

iClick™ EdU Andy Fluor™ 488 Flow Cytometry Assay Kit

Catalog Number:
CFA1277356ABP
Mfr. No.:
ABPB_A007
Price:
$640
  • Size:
    50 assays
    Quantity:
    Add to Cart:
      • Overview
        • Our iClick™ EdU Andy Fluor™ 488 Flow Cytometry Assay Kit provides a simplified, more robust assay for analyzing DNA replication in proliferating cells as compared to the traditional BrdU cell proliferation assay. In this assay, the modified thymidine analogue EdU is efficiently incorporated into newly synthesized DNA and fluorescently labeled with a bright, photostable Andy Fluor™ 488 dye in a fast, highly-specific click reaction. This fluorescent labeling of proliferating cells is accurate and compatible with antibody methods due to the mild click protocol. Newly synthesized DNA is analyzed using the 488 nm laser of the flow cytometer.

          Features
          • Simple: Labeling is complete in two steps
          • Accurate: Superior results compared to BrdU assay
          • Fast: Results in as little as 90 minutes
          • Multiplex: Compatible with cell cycle dyes and antibody-based stains
          • Quick and Simple Protocol

          Please contact us at for specific academic pricing.

          More Details

      • Properties
        • Categories
          Cell Proliferation Assay
          Other Properties
          Excitation/Emission (nm): 495/520

          * For research use only

      • Applications
        • Application
          Fluorescent
          Application Description
          Cell proliferation analysis by flow cytometer.
      • Reference
        • A chemical method for fast and sensitive detection of DNA synthesis in vivo. Salic A, Mitchison TJ, Proc Natl Acad Sci U S A (2008) 105:2415-2420
          Chick embryo proliferation studies using EdU labeling. Warren M, Puskarczyk K, Chapman SC, Dev Dyn (2009) 238:944-949
          Cell type specific applicability of 5-ethynyl-2′-deoxyuridine (EdU) for dynamic proliferation assessment in flow cytometry. Diermeier-Daucher S, Clarke ST, Hill D, Vollmann-Zwerenz A, Bradford JA, Brockhoff G, Cytometry A (2009) 75:535-546

    Documents

    Protocol-A007

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