Hygromycin B, Concentrated

- Overview
  - Hygromycin B is a unique aminoglycoside antibiotic derived from Streptomyces hygroscopicus. Hygromycin B is routinely used as a selective agent in cell culture or microbiology applications to isolate hygromycin B resistant cells.
    This product is packaged as a concentrated solution containing 350-450 units/mL.
    This product is considered a dangerous good. Quantities above 1 g may be subject to additional shipping fees. Please contact us for specific questions.
    
    Please contact us at for specific academic pricing.
    
    Background
    
    Hygromycin B inhibits protein synthesis by strengthening the interaction of tRNA binding in the ribosomal A-site. Hygromycin B also prevents mRNA and tRNA translocation by an unknown mechanism. These are unique mechanisms for an aminoglycoside antibiotic and they differ from the mode of action neomycin, gentamicin, and G418.
- Properties
  - CAS Number
    
    31282-04-9
    
    Molecular Formula
    
    C20H37N3O13
    
    Molecular Weight
    
    527.52 g/mol
    
    Appearance
    
    Amber or Brownish Solution
    
    Other Properties
    
    Source: Biosynthetic: produced by Streptomyces hygroscopicus.
    
    Storage
    
    Stock solutions should be stored at 2-8 °C.
    
    * For research use only
- Applications
  - Application Description
    
    Spectrum: Hygromycin B is effective against eukaryotic and prokaryotic cells.
    
    Microbiology Applications: Hygromycin B can be used as a selection agent to isolate hygromycin b resistant bacteria and fungi.
    
    Eukaryotic Cell Culture Applications: Hygromycin B is routinely used as a selective agent in mammalian cell culture to isolate hygromycin B resistant cells after transfection. Selectable markers for hygromycin B resistant cells include the hyg or hph resistance genes which express a phosphotransferase that inactivates hygromycin B by phosphorylation. Effective working concentrations range from 100 – 1000 µg/mL.For additional information regarding relevant cell lines, resistance plasmids, and culture media, please visit our cell culture database.