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Overview
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Hygromycin B binds near the A site of 16S rRNA and induces errors in the code by inhibition of translocation of peptydil-tRNA, however to a lesser extent compared to other aminoglycosides. Hygromycin B is efficient against bacteria, fungi and other eukaryotic cells, but more often used for the selection of eukaryotic cells including yeasts, such as, P. pastoris and S. cerevisiae, and mammalian cell lines, such as, CHO.
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