Human TNF-α Precoated ELISPOT Kit

- Overview
  - The kit includes detection antibody, streptavidin-HRP, AEC coloring system, dilution buffer, Cell stimulator , washing buffer and multifilter plate that is pre-coated with captured antibody. The kit is a ready-to-use and quality assurance product for all needs of ELISPOT assay. The performance of the kit is carefully examined to other comparable ELISPOT kits world wide to assure equivalent sensitivity, stability, and reproducibility.
    
    Please contact us at for specific academic pricing.
- Properties
  - Details
    
    Biotinylated antibody: 100μL
    Streptavidin-HRP: 100μL
    Cell stimulator: 50T
    Dilution buffer R (1×): 10mL×2
    Washing buffer (50×): 15mL
    AEC dilution: 10mL
    AEC solutionⅠ (20×): 500μL
    AEC solutionⅡ (20×): 500μL
    AEC solution Ⅲ (200×): 50μL
    Precoated PVDF plate: 1 plate
    
    Storage
    
    Store the unopened kit at 2-8°C. The kit is stable for 12 months. Do not use beyond expiration. Keep sterile once opened.
    
    * For Research Use Only.
- Reference
  - Liu,et al.[J] Chinese Journal of Immunology,2005,21(5):367-368
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    Zhou,et al.[J] Chinese Journal of Immunology,2007,12
    Xiao,et al.[J] Journal of Cellular and Molecular Immunology, 2007, 23(12): 1130-1132,1135
    Xu,et al.[J] Chinese Journal of Bilogicals, 2007,20(12): 907-909
    Dong,et al.[J] Journal of Modern Food and Pharmaceuticals,2007,17(4):3-5
    Liu,et al.[J] Chinese Journal of Pathophysiology,2007,23(1):99-102
    Yuan,et al.[J] Chinese Pharmaceutical Journal,2007,42(13):998-1002
    Zhang,et al.[J] Chinese Journal of Microbiology and Immunology,2007 ;27(11):1059-1060
    Xian-Hua Wang, et,al.[J]World J Gastroenterol,2005;11(36):5614-5620
    Xu-Dong Tang, et,al.[J]Cancer Research,2008,68(5):1529–1537
    Ting Chen,Xu-Dong Tang, et,al. [J] Neoplasia;2008(10):977–986.
    Ling Chen,Xu-Dong Tang,et,al. [J] J Pathol. 2009 Apr; 217(5):685-92.
    Gustafsson C, Hummerdal P, Matthiesen L, et al. Cytokine secretion in decidual mononuclear cells from term human pregnancy with or without labour: ELISPOT detection of IFN-gamma, IL-4, IL-10, TGF-beta and TNF-alpha.[J]. Journal of Reproductive Immunology, 2006, 71(1):41-56.
    Bailey T, Stark S, Grant A, et al. A multidonor ELISPOT study of IL-1β, IL-2, IL-4, IL-6, IL-13, IFN-γ and TNF-α release by cryopreserved human peripheral blood mononuclear cells[J]. Journal of Immunological Methods, 2002, 270(2):171-182.
    Yang L, Wang Y, Shi S, et al. The TNF-α induced expression of miR‐130b protects cervical cancer cells from the cytotoxicity of TNF-α[J]. Febs Open Bio, 2018, 8(4):614-627.

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Human TNF-α Precoated ELISPOT Kit

Human TNF-α Precoated ELISPOT Kit

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