Name: Human IFN-γ/IL-1β FluoroSpot Kit
Price: 851.00 USD

- Overview
  - The dual-color FluoroSpot assay is a modification of the T cell ELISPOT assay and developed for the simultaneous detection of two cytokines released by a single T cell. The assay is based on the use of antibodies labeled with different fluorophores, allowing the detection of T cells producing two pre-selected cytokines. The Fluorospot assay is particularly useful for the analysis of T cell subpopulations with distinct cytokine profiles.
    
    Characteristics: IFN-γ (green) and IL-1β (red)
    
    Please contact us at for specific academic pricing.
- Properties
  - Details
    
    • Coating antibodies
    • Biotinylated detection antibodies
    • Fluorescent-labeled conjugate
    • Blocking stock solution
    • Dilution buffer R
    • Spot enhancer
    • Tween-20
    • PVDF FluoroSpot plates with lids and cover slips
    • Manual
    
    * For research use only.
- Applications
  - Application Description
    
    • The FluoroSpot assay is an effective tool with a high sensitivity to enumerate antigen-specific T cells with simultaneous detection of two different cytokines in the circulation of (non)immunized humans and animals.
    • Since the FluoroSpot assay is able to detect 2 cytokines simultaneously in one assay, the necessary amount of PBMCs required is lower. Therefore, the Fluorospot is an ideal tool for clinical trials with a limited amount of PBMCs.
    • The FluoroSpot assay can be used to evaluate T cell responses in individuals with type 1 diabetes or at risk of it.

Documents

SDS

Manual

Human IFN-γ/IL-1β FluoroSpot Kit

Human IFN-γ/IL-1β FluoroSpot Kit

Documents

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