HT29-cas9 Cell (Human Colon Cancer Cell)

Species: human
Cell Morphology: Adherent cells, epithelial-like
Complete Culture Medium Components: McCoy 5A+10%FBS
Mycoplasma testing: Negative
Antibiotic Concentration: puromycin, 1 ug/ml
Cultivation Environment: 37°C, 5% CO₂
Passage Ratio: 1/3 to 1/4 of the offspring are passed down.
Frequency of Reproduction: 2-3 times/week
Gene Expression: Cas9 protein, spCas9, CRISPR-Cas9 protein

Product Advantages:
• Efficiency and Convenience: A "ready-to-use" platform; simply import gRNA, operation is simple, editing efficiency is high, saving time and costs.
• High-Throughput Screening: An ideal foundational platform for genome-wide CRISPR screening, used for systematic discovery of functional genes.
• Consistency and Reproducibility: Consistent cell population genetic background, stable Cas9 expression, more reliable experimental results, and more rigorous control settings.
• Functional Scalability: Cell lines expressing dCas9, high-fidelity Cas9, and other variants can be constructed to achieve multiple functions beyond gene knockout (regulation, imaging, etc.).

Please contact us at for specific academic pricing.

Background

HT-29 cells were isolated from primary tumors by J. Fogh in 1964 using a transplantation culture method and F12 medium containing 15% FBS; recently, established cell lines have been cultured using McCoy's 5A medium containing the same serum. HT-29 cells induce tumors in nude mice and also in steroid-treated hamsters. HT-29 cells synthesize IgA, CEA, protein-bound secretory components of TGF-β, and mucin, among other things.