HotBegan™ Hot Start Red DNA Polymerase MasterMix

For a Specific, Highly Efficient & Reliable Amplifications of DNA up 160 fg, with visual Tracking of DNA Migration

HotBegan™ Hot Start Red DNA Polymease Master Mix (2x) is an optimized ready-to-use solution containing HotBegan™ Hot Start Taq DNA Polymerase, dNTPs, MgCl2 and Stabilizers. It is inactive at room temperature and only requires addition of template, primers, and water.

HotBegan™ Hot Start Taq DNA Polymerase is a Taq DNA Polymerase bound to a proprietary antibody that blocks Polymerase activity until denaturation step occurs. The heat labile antibodies are rapidly inactivated by raising the temperature (4 minutes at 95-97 °C). This prevents or minimizes primer-dimer and nonspecific products.

MasterMix also contains an Agarose Loading Buffer including a Red Dye for visual tracking of DNA migration and a dense compound to facilitate the drop-down of the samples into the well Agarose Gels. Like the Taq polymerase, the enzyme has 5'→3' polymerase activity and a weak 5'→3' exonuclease activity but no 3'→5' exonuclease activity (proofreading). Before enzyme activation none of enzyme activities are detectable.

Advantages & Features
▪ Accurate & Fast visual Tracking of DNA Migration: thanks to its composition that includes Red Dye Buffer.
▪ High specificity: minimizes unspecific amplification.
▪ Efficient: prevents or minimizes primer-dimer and nonspecific products.
▪ Great sensitivity: amplifies from femptograms of DNA targets.
▪ Inactive: at Room Temperature.
▪ Optimized
▪ Powerful: amplification of targets up to 5 kb.
▪ Convenient: available in kits and MasterMix solution.

Specifications
▪ Assay Conditions: 25mM Tris-HCl pH9.0 at 25°C, 50mM KCl, 2mM MgCl2, 0.1mg/mL gelatine, 200 µM of dATP, dGTP, dTTP, 100µM[α32-P]dCTP (0.05µCi/nmol) and 12.5 µg activated salmon sperm DNA.
▪ Unit Definition: One unit is defined as the amount of enzyme required to catalyse the incorporation of 10 nanomoles of dNTPs into acid-insoluble material in 30 minutes at 74 °C.

Includes for 250 rxn:
– 5 x 1.25 mL HotBegan™ Hot Start Red DNA Polymerase MasterMix (2x)*
– 1.5 mL MgCl2 Solution (50mM)**
* Includes HotBegan™ Hot Start Taq DNA Polymerase, Red Dye buffer (2x), 0.4 mM of each dNTP, 5 mM Mg2+ and 5% Glycerol.
**Separate tube 50 mM MgCl2 solution is provided for further optimisation. In some cases, we recommend to optimize Mg2 + concentration.

Quality Control
▪ Functionally tested in PCR.
▪ Exempt of nucleases (endo-, exo and ribonucleases) activities guaranteed by appropriate quality tests.

Please contact us at for specific academic pricing.