Name: Horse-Power™ Red DNA Polymerase MasterMix
Price: 203.00 USD

- Overview
  - For an Optimized, Accurate & Fast visual Tracking of DNA Migration
    
    Horse-Power™ Red DNA Polymerase is an optimized, accurate and ready-to-use MasterMix (2.5x) that incorporates all PCR reaction components: TruePure™ dNTPs, PCR buffer, Mg²⁺ and Horse-Power™ Taq DNA Polymerase.
    The mix also incorporates an agarose Loading Buffer including a red dye for visual tracking of DNA migration and a dense compound to facilitate the drop-down of the samples into the well agarose gels.
    
    Advantages & Features
    ▪ Optimized: adds extra nucleotides (preferentially adenine) without template at 3´ends leaving 3´overhangs PCR fragments.
    ▪ Time-saving: ready-to-use format that saves time in PCR process and in loading samples on agarose gels.
    ▪ Complete solution: includes all PCR reaction components except primers and template.
    
    Specifications
    ▪ Concentration: 2.5x (Buffer Red 2.5X, TruePure™ dNTPs 0.5 mM each, HorsePower™ Taq DNA Polymerase 0.25 U/μL, Glycerol 30%).
    ▪ Assay conditions: 25 mM Tris-HCl pH9.0 at 25 °C, 50 mM KCl, 2 mM MgCl2, 0.1 mg/mL gelatine, 200 µM dATP, dGTP, dTTP, 100 µM [α32-P] dCTP (0.05 µCi/nmol) and 12.5 µg activated salmon sperm DNA.
    
    Includes
    – 100 rxn Horse-Power™ Red DNA Polymerase Master Mix (2.5x)
    *Includes all necessary reagents except template and primer DNA.
    
    Quality Control
    ▪ Functionally tested in PCR.
    ▪ Free of bacterial DNA (by qPCR).
    ▪ Exempt of nucleases (endo-, exo and ribonucleases) activities guaranteed by appropriate quality tests.
    
    Please contact us at for specific academic pricing.
- Properties
  - Storage
    
    Gel pack
    
    Shipping
    
    -20 °C
    
    * This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO).
- Applications
  - Application Description
    
    Design for medium or high throughput applications (e.g. colony screening).
    PCR fragments amplification for TA or GC cloning.
- Reference
  - Angelova, G., Stefanova, P., Brazkova, M., & Krastanov, A. (2023). Molecular and morphological characterization of Xylaria karsticola (Ascomycota) isolated from the fruiting body of Macrolepiota procera (Basidiomycota) from Bulgaria. Plos one, 18(6), e0287679.
    Petrova, S., & Petkova, M. (2023). Plant Traits of Tilia tomentosa Moench, Fraxinus excelsior L., and Pinus nigra JF Arnold as a Proxy of Urbanization. Forests, 14(4), 800.
    Petkova, M., Gotcheva, V., Dimova, M., Bartkiene, E., Rocha, J. M., & Angelov, A. (2022). Screening of Lactiplantibacillus plantarum Strains from Sourdoughs for Biosuppression of Pseudomonas syringae pv. syringae and Botrytis cinerea in Table Grapes. Microorganisms, 10(11), 2094.
    Petkova, M., Spasova-Apostolova, V., Masheva, V., Atanasova, D., & Tahsin, N. (2021). Endophytic colonization of Solanaceае family plants by fungal entomopathogen Beauveria bassiana strain 339 to control Colorado potato beetle (Leptinotarsa decemlineata Say). Bulgarian Journal of Agricultural Science, 27, 1.
    Petkova, Mariana, et al. “Isolation and Characterization of Lactic Acid Bacteria and Yeasts from Typical Bulgarian Sourdoughs.” Microorganisms 9.7 (2021): 1346.
    Petkova, Mariana, et al. “MICROBIOLOGICAL AND PHYSICOCHEMICAL CHARACTERIZATION OF TRADITIONAL BULGARIAN SOURDOUGHS AND SCREENING OF LACTIC ACID BACTERIA FOR AMYLOLYTIC ACTIVITY.” Journal of Chemical Technology and Metallurgy 55.5 (2020): 921-934.
    Ruiz Carrascoso, G. (2016). Características microbiológicas y clínico-epidemiológicas de enterobacterias productoras de carbapenemasa oxa-48 en el contexto de un brote hospitalario (Doctoral dissertation, Universidad Complutense de Madrid).

Datasheet

Horse-Power™ Red DNA Polymerase MasterMix

Horse-Power™ Red DNA Polymerase MasterMix

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