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HotBegan™ Hot Start Taq DNA Polymerase MasterMix

HotBegan™ Hot Start Taq DNA Polymerase MasterMix

Catalog Number:

PER1268501CAN

Mfr. No.:

P0030

Price:

$232

Size:

2 x 1.25 mL (250 rxn)

Quantity:

Add to Cart:

- Overview
  - HotBegan™ Hot Start Taq DNA Polymerase MasterMix (2x) an optimized ready-to-use solution containing HotBegan™ Taq DNAPolymerase (hot start performance), dNTPs, MgCl2 and Stabilizers. It is inactive at Room Temperature and includes all material needed except Template and Primers. It is a Horse-Power™ Taq DNA Polymerase bound to a proprietary antibody that blocks polymerase activity until a denaturation step occurs. The heat-labile antibodies are rapidly inactivated by raising the temperature (4 minutes at 95-97 °C). This prevents or minimizes primer-dimer and non-specific products. Like Horse-Power™ Taq DNA Polymerase, the enzyme has 5’→3’ polymerase activity and a weak 5’→3’ exonuclease activity but no 3’→5’ exonuclease activity (proofreading). Before enzyme activation none of the enzyme activities are detectable.
    
    Includes:
    – 2 x 1. 25 mL HotBegan™ Hot Start Taq DNA Polymerase MasterMix, which includes HotBegan™ Hot Start Taq DNA Polymerase (0. 1 U/μL), 2x PCR Buffer, 0. 4 mM of each dNTP, 4 mM Mg2+, 4% Glycerol. – 2 x 1. 25 mL Nuclease-free water.
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    Hot Start PCR
    
    Storage
    
    -20 °C.
    
    * This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals.
- Applications
  - Application
    
    Real-Time PCR (qPCR)
    
    Application Description
    
    Real time PCR.
    RT-PCR and quantitative RT-PCR.
    Genotyping with Taqman Probes.
    PCR fragments amplification for TA or GC Cloning.
    Amplification from a limited DNA template or low copy number genes.
- Reference
  - Albuquerque, C. A. D. (2019). Detection and characterization of Dichelobacter nodosus from sheep with different clinical manifestations of Ovine Footrot (Doctoral dissertation).
    Osset Iborra, H. (2019). Seguimiento de las plagas de Tomicus destruens y Orthotomicus erosus y detección de Botryosphaeriaceae, hongos patógenos asociados a estos insectos, en los pinares de Pinus halepensis de La Pedrera y la Devesa del Saler (Valencia) (Doctoral dissertation).
    Negreiros, A. M., Júnior, R. S., Rodrigues, A. P., León, M., & Armengol, J. Prevalent weeds collected from cucurbit fields in Northeastern Brazil reveal new species diversity in the genus Monosporascus. Annals of Applied Biology.
    Dejene, T., Oria-de-Rueda, J. A., & Martín-Pinto, P. (2017). Fungal community succession and sporocarp production following fire occurrence in Dry Afromontane forests of Ethiopia. Forest Ecology and Management, 398, 37-47.
    Criado-García, J., Fuentes, F., Cruz-Teno, C., García-Rios, A., Jiménez-Morales, A., Delgado-Lista, J., … & Pérez-Jiménez, F. (2011). R353Q polymorphism in the factor VII gene and cardiovascular risk in Heterozygous Familial Hypercholesterolemia: a case-control study. Lipids in health and disease, 10(1), 50.
    Català, S., Pérez-Sierra, A., & Abad-Campos, P. (2015). The use of genus-specific amplicon pyrosequencing to assess Phytophthora species diversity using eDNA from soil and water in northern Spain. PloS one, 10(3), e0119311.
    Olmo, D., Armengol, J., León, M., & Gramaje, D. (2016). Characterization and Pathogenicity of Botryosphaeriaceae Species Isolated from Almond Trees on the Island of Mallorca (Spain). Plant Disease, 100(12), 2483-2491.
    Dejene, T., Oria-de-Rueda, J. A., & Martín-Pinto, P. (2017). Fungal diversity and succession following stand development in Pinus patula Schiede ex Schltdl. & Cham. plantations in Ethiopia. Forest Ecology and Management, 395, 9-18.

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