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Overview
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Horse-Power™ Taq DNA Polymerase is a pure, versatile and thermostable recombinant enzyme produced in an E. coli strain, which carries the cloned pol gene from Thermus aquaticus. The enzyme has 5’→3’ polymerase activity and a weak 5’→3’ exonuclease activity but no 3’→5’ exonuclease activity (proofreading). GMP-grade reagent also available. Learn more.
Concentration: 5 U/μL – Includes for 500 U: – 100 μL Horse-Power™ Taq Polymerase (5 U/μL) – 25 mM MgCl2 (1. 5 mL) – 1. 5 mL Buffer (10x)
Concentration: 1 U/μL – Includes for 500 U:
– 500 μL Horse-Power™ Taq Polymerase (1 U/μL) – 25 mM MgCl2 (1. 5 mL) – 1. 5 mL Buffer (10x)Please contact us at for specific academic pricing.
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Properties
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CategoriesRoutine PCRStorage-20 °C (NON Frost-Free Freezer).
* This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals.
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Applications
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ApplicationEnd-Point PCRApplication DescriptionRoutine amplifications.
Colony screening (see Horse-Power™ Red-Taq DNA Polymerase).
Amplifications up to 5 kb using plasmid, viral or genomic DNA as template.
PCR fragments amplification for TA or GC cloning (preferably use a proofreading polymerase for cloning purposes combined with an efficient blunt cloning vector) (see pSpark® DNA Cloning System).
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Reference
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Sohrabi, M., Mohammadi, H., Armengol, J., & León, M. (2022). New report of Biscogniauxia rosacearum as a pathogen on almond trees in Iran. Journal of Plant Diseases and Protection, 1-7.
Villa Suárez, J. M. (2022). Estudio poblacional de hipofosfatasia en la provincia de Granada y caracterización funcional y estructural de las variantes genéticas identificadas.
Aguilar, Lorena, et al. «Effect of intermittent induced aeration on nitrogen removal and denitrifying-bacterial community structure in Cork and gravel vertical flow pilot-scale treatment wetlands.» Journal of Environmental Science and Health, Part A (2021): 1-10.
Porcellato, Ilaria, et al. «PD-L1/PD-1 and CTLA-4 Expression in Equine Penile Squamous Cell Carcinomas.» Animals 11.7 (2021): 2121.
Scarlet, D., Handschuh, S., Reichart, U., Podico, G., Ellerbrock, R. E., Demyda-Peyras, S., … & Aurich, C. (2021). Sexual Differentiation and Primordial Germ Cell Distribution in the Early Horse Fetus.
León, M., Berbegal, M., Abad-Campos, P., Ramón-Albalat, A., Caffi, T., Rossi, V., … & Armengol, J. (2021). Evaluation of Sown Cover Crops and Spontaneous Weed Flora as a Potential Reservoir of Black-Foot Pathogens in Organic Viticulture. Biology, 10(6), 498.
Abdullah, Y. S., Abdullah, S. M. A., & Hussein, R. H. (2021). Ultramorphology and molecular studies of Contracaecum larvae (Nematoda: Anisakidae) collected in five Cyprinid fish species from Sulaimani Province, Kurdistan Region-Iraq. Helminthologia, 58(1), 41-58.
Abdullah, Y. S., Abdullah, S. M., & Hussein, R. H. (2021). Morphology and Molecular Studies of Contracaecum Larvae (Nematoda: Anisakidae) in Some Fish species from Sulaimani Province, Kurdistan Region, Iraq. Basrah J. Agric. Sci., 34(1), 93-110.
Miranda-Fuentes, P., García-Carneros, A. B., & Molinero-Ruiz, L. (2021). Updated Characterization of Races of Plasmopara halstedii and Entomopathogenic Fungi as Endophytes of Sunflower Plants in Axenic Culture. Agronomy, 11(2), 268.
Jiménez Muñoz, R. (2020). Desarrollo de herramientas fisiológicas y genómicas para mejorar la calidad postcosecha del fruto de calabacín.
Sunesen, I., Rodríguez Hernández, F., Aguiar Juárez, D., Tardivo Kubis, J. A., Lavigne, A. S., Rossignoli, A., … & Sar, E. A. (2020). Morphology, genetics and toxin profile of Prorocentrum texanum (Dinophyceae) from Argentinian marine coastal waters. Phycologia, 1-17.
Arjona-López, J. M., & López-Herrera, C. J. (2020). Entoleuca sp. infected by mycoviruses as potential biocontrol agents of avocado white root rot. European Journal of Plant Pathology, 1-12.
Porcellato, I., Mecocci, S., Mechelli, L., Cappelli, K., Brachelente, C., Pepe, M., … & Modesto, P. (2020). Equine Penile Squamous Cell Carcinomas as a Model for Human Disease: A Preliminary Investigation on Tumor Immune Microenvironment. Cells, 9(11), 2364.
Hatzilazarou, S., Kostas, S., Joachim, M., & Economou, A. (2020). Regeneration of Viburnum dentatum L. from Alginate-Encapsulated Shoot Explants after Short-Term Cold Storage and Assessment of Genetic Stability Using ISSR Analysis. Agronomy, 10(11), 1660.
Díaz, P. A., Fernández-Pena, C., Pérez-Santos, I., Baldrich, Á., Díaz, M., & Rodríguez, F. (2020). Dinophysis Ehrenberg (Dinophyceae) in Southern Chile harbours red cryptophyte plastids from Rhodomonas/Storeatula clade. Harmful Algae, 99, 101907.
RUSENOVA, N., KRUSTEV, S., ATANASOV, A., RUSENOV, A., & STANILOVA, S. Differentiation of Staphylococcus pseudintermedius in the Staphylococcus intermedius Group (SIG) by Conventional and Molecular Methods. thought, 10, 11.
Miranda‐Fuentes, P., García‐Carneros, A. B., Montilla‐Carmona, A. M., & Molinero‐Ruiz, L. Evidence of soil‐located competition as the cause of the reduction of sunflower Verticillium wilt by entomopathogenic fungi. Plant Pathology.
Gilannejad, Neda, et al. «Ontogeny of Expression and Activity of Digestive Enzymes and Establishment of gh/igf1 Axis in the Omnivorous Fish Chelon labrosus.» Animals 10.5 (2020): 874.
García-Girón, J., García, P., Fernández-Aláez, M., Bécares, E., & Fernández-Aláez, C. (2019). Bridging population genetics and the metacommunity perspective to unravel the biogeographic processes shaping genetic differentiation of Myriophyllum alterniflorum DC. Scientific Reports, 9(1), 1-10.
Robledo Mahón, T. (2018). Estudio de los procesos biológicos y de la estructura de las comunidades microbianas en el proceso de compostaje de lodos de depuradora de aguas residuales urbanas, en sistemas de membranas semipermea.
Arjona-López, J. M., Capote, N., & López-Herrera, C. J. (2019). Improved real-time PCR protocol for the accurate detection and quantification of Rosellinia necatrix in avocado orchards. Plant and Soil, 1-8.
Fagín, E., Bravo, I., Garrido, J. L., Rodríguez, F., & Figueroa, R. I. Scrippsiella acuminata versus Scrippsiella ramonii: A Physiological Comparison. Cytometry Part A.
Ortiz‐Bustos, C. M., López‐Bernal, A., Testi, L., & Molinero‐Ruiz, L. Environmental and irrigation conditions can mask the effect of Magnaporthiopsis maydis on growth and productivity of maize. Plant Pathology.
SERRA MARTÍNEZ,. (2017). Detección de posibles fuentes de inoculo de Fusicladium eriobotryae en níspero.
Suarez‐Bregua, P., Chien, C. J., Megias, M., Du, S., & Rotllant, J. (2017). Promoter architecture and transcriptional regulation of musculoskeletal embryonic nuclear protein 1b (mustn1b) gene in zebrafish. Developmental Dynamics, 246(12), 992-1000.
Ruiz-Ruano, F. J., Castillo-Martínez, J., Cabrero, J., Gómez, R., Camacho, J. P. M., & López-León, M. D. (2018). High-throughput analysis of satellite DNA in the grasshopper Pyrgomorpha conica reveals abundance of homologous and heterologous higher-order repeats. Chromosoma, 127(3), 323-340.
Aguilar, L., Gallegos, Á., Arias, C. A., Ferrera, I., Sánchez, O., Rubio, R., … & Pérez, C. (2019). Microbial nitrate removal efficiency in groundwater polluted from agricultural activities with hybrid cork treatment wetlands. Science of the total environment, 653, 723-734.
Robledo-Mahón, T., Silva-Castro, G. A., Kuhar, U., Jamnikar-Ciglenečki, U., Barlič-Maganja, D., Aranda, E., & Calvo, C. (2019). Effect of Composting Under Semipermeable Film on the Sewage Sludge Virome. Microbial ecology, 1-9.
Negreiros, A. M. P., Sales Júnior, R., León, M., Melo, N. J. D. A., Michereff, S. J., Ambrósio, M. M. D. Q., … & Armengol, J. Identification and pathogenicity of Macrophomina species collected from weeds in melon fields in Northeastern Brazil. Journal of Phytopathology.
Rêgo, T. J. S., Elena, G., Correia, K. C., Tovar‐Pedraza, J. M., Câmara, M. P. S., Armengol, J., … & Berbegal, M. Genetic diversity and population structure of Lasiodiplodia theobromae from different hosts in northeastern Brazil and Mexico. Plant Pathology.
Flores, B., & Javier, F. (2018). Diagnóstico de enfermedades causadas por hongos en plantas de palmera y tipo palmera de uso ornamental, en un vivero de la Comunidad Valenciana.
Robledo Mahón, T. (2018). Estudio de los procesos biológicos y de la estructura de las comunidades microbianas en el proceso de compostaje de lodos de depuradora de aguas residuales urbanas, en sistemas de membranas semipermea.
Mora-Sala, B., Cabral, A., León, M., Agustí-Brisach, C., Armengol, J., & Abad-Campos, P. Survey, Identification and Characterization of cylindrocarpon-like asexual morphs in Spanish forest nurseries. Plant Disease, (ja).
Robledo-Mahón, T., Aranda, E., Pesciaroli, C., Rodríguez-Calvo, A., Silva-Castro, G. A., González-López, J., & Calvo, C. (2018). Effect of semi-permeable cover system on the bacterial diversity during sewage sludge composting. Journal of Environmental Management, 215, 57-67.
Ruiz-Ruano, F. J., Castillo-Martínez, J., Cabrero, J., Gómez, R., Camacho, J. P. M., & López-León, M. D. (2018). High-throughput analysis of satellite DNA in the grasshopper Pyrgomorpha conica reveals abundance of homologous and heterologous higher-order repeats. Chromosoma, 1-18.
Núñez Delegido, E. (2017). Caracterización funcional de los genes CRD de Arabidopsis thaliana.
Ruiz-Estévez, M., Bakkali, M., Martín-Blázquez, R., & Garrido-Ramos, M. A. (2017). Differential expression patterns of MIKCC-type MADS-box genes in the endangered fern Vandenboschia speciosa. Plant Gene, 12, 50-56.
Rodriguez-Sanchez, A., Margareto, A., Robledo-Mahon, T., Aranda, E., Diaz-Cruz, S., Gonzalez-Lopez, J., … & Gonzalez-Martinez, A. (2017). Performance and bacterial community structure of a granular autotrophic nitrogen removal bioreactor amended with high antibiotic concentrations. Chemical Engineering Journal.
Anaya Calvo-Rubio, G. (2017). Desarrollo y validación de un sistema diagnóstico rápido para la detección de alteraciones cromosómicas que provocan infertilidad en caballos basado en herramientas moleculares.
Calvo‐Polanco, M., Sánchez‐Castro, I., Cantos, M., García, J. L., Azcón, R., Ruiz‐Lozano, J. M., … & Aroca, R. (2016). Effects of different arbuscular mycorrhizal fungal backgrounds and soils on olive plants growth and water relation properties under well‐watered and drought conditions. Plant, Cell & Environment, 39(11), 2498-2514.
Núñez Delegido, E. (2017). Caracterización funcional de los genes CRD de Arabidopsis thaliana.
León-Ortega, M., & González-Wangüemert, M. (2015). Characterization of 10 new tetranucleotide microsatellite markers for the European eagle owl, Bubo bubo: Useful tools for conservation strategies. Biochemical Systematics and Ecology, 63, 109-112.
Demyda-Peyrás, S., Anaya, G., Bugno-Poniewierska, M., Pawlina, K., Membrillo, A., Valera, M., & Moreno-Millán, M. (2014). The use of a novel combination of diagnostic molecular and cytogenetic approaches in horses with sexual karyotype abnormalities: a rare case with an abnormal cellular chimerism. Theriogenology, 81(8), 1116-1122.
Navarro-Domínguez, B., Cabrero, J., Camacho, J. P. M., & López-León, M. D. (2016). B-chromosome effects on Hsp70 gene expression does not occur at transcriptional level in the grasshopper Eyprepocnemis plorans. Molecular Genetics and Genomics, 291(5), 1909-1917.
ALMENDROS, V. M. T. (2016). Identificación de especies de Dactylonectria e Ilyonectria asociadas al pie negro de la vid en Castilla la Mancha.
Martínez-Miguel, P., Valdivielso, J. M., Medrano-Andrés, D., Román-García, P., Cano-Peñalver, J. L., Rodríguez-Puyol, M., … & López-Ongil, S. (2014). The active form of vitamin D, calcitriol, induces a complex dual upregulation of endothelin and nitric oxide in cultured endothelial cells. American Journal of Physiology-Endocrinology and Metabolism, 307(12), E1085-E1096.
Daddaoua, A., Molina-Santiago, C., de la Torre, J., Krell, T., & Ramos, J. L. (2014). GtrS and GltR form a two-component system: the central role of 2-ketogluconate in the expression of exotoxin A and glucose catabolic enzymes in Pseudomonas aeruginosa. Nucleic acids research, 42(12), 7654-7665.
Nieves-Morión, M., Lechno-Yossef, S., López-Igual, R., Frías, J. E., Mariscal, V., Nürnberg, D. J., … & Flores, E. (2017). Specific glucoside transporters influence septal structure and function in the filamentous, heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120. Journal of Bacteriology, JB-00876.
Sierra, A. M. P., Sala, B. M., Santana, M. L., Jiménez, J. G., & Campos, P. A. (2012). Enfermedades causadas por en viveros de plantas ornamentales. In Boletin de Sanidad Vegetal: Plagas (Vol. 38, No. 1, pp. 143-156). Ministerio de Agricultura, Pesca y Alimentacion, Gobierno de España.
Pérez-Sierra, A., Landeras, E., León, M., Berbegal, M., García-Jiménez, J., & Armengol, J. (2007). Characterization of Fusarium circinatum from Pinus spp. in northern Spain. Mycological research, 111(7), 832-839.
Valverde-Tercedor, C., Montalbán-López, M., Perez-Gonzalez, T., Sanchez-Quesada, M. S., Prozorov, T., Pineda-Molina, E., … & Jimenez-Lopez, C. (2015). Size control of in vitro synthesized magnetite crystals by the MamC protein of Magnetococcus marinus strain MC-1. Applied microbiology and biotechnology, 99(12), 5109-5121.
Mohammadi, H., Banihashemi, Z., Gramaje, D., & Armengol, J. (2012). Fungal pathogens associated with grapevine trunk diseases in Iran. Journal of Agricultural Science and Technology, 15(1), 137-150.
Hernández-Hernández, J., Espino, A., Rodríguez-Rodríguez, J. M., Pérez-Sierra, A., León, M., Abad-Campos, P., & Armengol, J. (2010). Survey of diseases caused by Fusarium spp. on palm trees in the Canary Islands. Phytopathologia Mediterranea, 49(1), 84-88.
Boughalleb, N., Salem, I. B., Beltrán, R., Vicent, A., Sierra, A. P., Abad‐Campos, P., … & Armengol, J. (2010). Occurrence of Monosporascus cannonballus in watermelon fields in Tunisia and factors associated with ascospore density in soil. Journal of phytopathology, 158(3), 137-142.
Soto, E. C., Yáñez-Ruiz, D. R., Cantalapiedra-Hijar, G., Vivas, A., & Molina-Alcaide, E. (2012). Changes in ruminal microbiota due to rumen content processing and incubation in single-flow continuous-culture fermenters. Animal production science, 52(9), 813-822.
Jroundi, F., Fernández-Vivas, A., Rodriguez-Navarro, C., Bedmar, E. J., & González-Muñoz, M. T. (2010). Bioconservation of deteriorated monumental calcarenite stone and identification of bacteria with carbonatogenic activity. Microbial ecology, 60(1), 39-54.
Macias, M. I., Grande, J., Moreno, A., Domínguez, I., Bornstein, R., & Flores, A. I. (2010). Isolation and characterization of true mesenchymal stem cells derived from human term decidua capable of multilineage differentiation into all 3 embryonic layers. American journal of obstetrics and gynecology, 203(5), 495-e9.
Clemente, A., Marín-Manzano, M. C., Jiménez, E., Arques, M. C., & Domoney, C. (2012). The anti-proliferative effect of TI1B, a major Bowman–Birk isoinhibitor from pea (Pisum sativum L.), on HT29 colon cancer cells is mediated through protease inhibition. British Journal of Nutrition, 108(S1), S135-S144.
Alvarez, L. A., León, M., Abad-Campos, P., García-Jiménez, J., & Vicent, A. (2011). Genetic variation and host specificity of Phytophthora citrophthora isolates causing branch cankers in Clementine trees in Spain. European journal of plant pathology, 129(1), 103-117.
Pérez-Sierra, A., León, M., Álvarez, L. A., Alaniz, S., Berbegal, M., García-Jiménez, J., & Abad-Campos, P. (2010). Outbreak of a new Phytophthora sp. associated with severe decline of almond trees in eastern Spain. Plant Disease, 94(5), 534-541.
Jiménez-Hidalgo, M., Kurz, C. L., Pedrajas, J. R., Naranjo-Galindo, F. J., González-Barrios, M., Cabello, J., … & Fierro-González, J. C. (2014). Functional characterization of thioredoxin 3 (TRX-3), a Caenorhabditis elegans intestine-specific thioredoxin. Free Radical Biology and Medicine, 68, 205-219.
Berbegal, M., Pérez-Sierra, A., Armengol, J., & Grünwald, N. J. (2013). Evidence for multiple introductions and clonality in Spanish populations of Fusarium circinatum. Phytopathology, 103(8), 851-861.
Alaniz, S., Armengol, J., León, M., García-Jiménez, J., & Abad-Campos, P. (2009). Analysis of genetic and virulence diversity of Cylindrocarpon liriodendri and C. macrodidymum associated with black foot disease of grapevine. Mycological research, 113(1), 16-23.
Mohammadi, H., Alaniz, S., Banihashemi, Z., & Armengol, J. (2009). Characterization of Cylindrocarpon liriodendri associated with black foot disease of grapevine in Iran. Journal of Phytopathology, 157(10), 642-645.
Rafael, R., Márquez, M. C., Papke, R. T., & Ventosa, A. (2012). Multilocus sequence analysis of the family Halomonadaceae. International journal of systematic and evolutionary microbiology, 62(3), 520-538.
Ruiz, R., Barroso-Deljesus, A., Lara, L., & Rubio, L. A. (2015). The use of multiple restriction enzymes in terminal restriction fragment length polymorphism analysis and identification of performance-related caecal bacterial groups in growing broiler chickens. The Journal of Agricultural Science, 153(08), 1491-1505.
Dorado, J., Anaya, G., Bugno‐Poniewierska, M., Molina, A., Mendez‐Sanchez, A., Ortiz, I., … & Demyda‐Peyrás, S. (2016). First case of sterility associated with sex chromosomal abnormalities in a jenny. Reproduction in Domestic Animals.
García-Carneros, A. B., García-Ruiz, R., & Molinero-Ruiz, L. (2014). Genetic and Molecular Approach to Verticillium dahliae Infecting Sunflower. Helia, 37(61), 205-214.
Ortiz-Bustos, C. M., Testi, L., García-Carneros, A. B., & Molinero-Ruiz, L. (2016). Geographic distribution and aggressiveness of Harpophora maydis in the Iberian peninsula, and thermal detection of maize late wilt. European Journal of Plant Pathology, 144(2), 383-397.
Soto, E. C., Molina-Alcaide, E., Khelil, H., & Yáñez-Ruiz, D. R. (2013). Ruminal microbiota developing in different in vitro simulation systems inoculated with goats’ rumen liquor. Animal Feed Science and Technology, 185(1), 9-18.
Rodríguez-Ruano, S. M., Martín-Vivaldi, M., Martín-Platero, A. M., López-López, J. P., Peralta-Sánchez, J. M., Ruiz-Rodríguez, M., … & Martínez-Bueno, M. (2015). The Hoopoe’s uropygial gland hosts a bacterial community influenced by the living conditions of the bird. PloS one, 10(10), e0139734.
Armengol, J., Vicent, A., León, M., Berbegal, M., Abad‐Campos, P., & García‐Jiménez, J. (2010). Analysis of population structure of Rosellinia necatrix on Cyperus esculentus by mycelial compatibility and inter‐simple sequence repeats (ISSR). Plant pathology, 59(1), 179-185.
Pérez‐Sierra, A., López‐García, C., León, M., García‐Jiménez, J., Abad‐Campos, P., & Jung, T. (2013). Previously unrecorded low‐temperature Phytophthora species associated with Quercus decline in a Mediterranean forest in eastern Spain. Forest Pathology, 43(4), 331-339.
González‐Domínguez, E., León, M., Armengol, J., & Berbegal, M. (2015). A Nested Polymerase Chain Reaction Protocol for in planta Detection of Fusicladium eriobotryae, Causal Agent of Loquat Scab. Journal of Phytopathology, 163(5), 415-418.
Tello, M. L., Gramaje, D., Gómez, A., Abad-Campos, P., & Armengol, J. (2010). Analysis of phenotypic and molecular diversity of Phaeomoniella chlamydospora isolates in Spain. Journal of Plant Pathology, 195-203.
Salem, I. B., Correia, K. C., Boughalleb, N., Michereff, S. J., León, M., Abad-Campos, P., … & Armengol, J. (2013). Monosporascus eutypoides, a cause of root rot and vine decline in Tunisia, and evidence that M. cannonballus and M. eutypoides are distinct species. Plant disease, 97(6), 737-743.
González, M., Pérez‐Sierra, A., Serrano, M. S., & Sánchez, M. E. (2016). Two Phytophthora species causing decline of wild olive (Olea europaea subsp. europaea var. sylvestris). Plant Pathology.
Fernández-Bedmar, Z., & Alonso-Moraga, A. (2016). In vivo and in vitro evaluation for nutraceutical purposes of capsaicin, capsanthin, lutein and four pepper varieties. Food and Chemical Toxicology, 98, 89-99.
Pacheco Piñol, S., & Roig Navarro, I. (2015). The role of ATR kinase during mammalian gametogenesis.
Marcet Ortega, M., & Roig, I. (2016). Surveillance mechanisms in mammalian meiosis.
CRESPO, J. A. (2016). Efecto de los tratamientos por termoterapia con agua caliente sobre la micoflora presente en plantas de vid injertadas.
Agustí-Brisach, C., Cabral, A., González-Domínguez, E., Pérez-Sierra, A., León, M., Abad-Campos, P., … & Armengol, J. (2016). Characterization of Cylindrodendrum, Dactylonectria and Ilyonectria isolates associated with loquat decline in Spain, with description of Cylindrodendrum alicantinum sp. nov. European Journal of Plant Pathology, 145(1), 103-118.
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Overview