HiPure Circulating DNA Midi Spin Kit D (Spin Protocol)

Free-circulating nucleic acids, such as tumor-specific extracellular DNA fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasmausually as short fragments, <1000bp(DNA). HiPure Circulating DNA Midi Kit enables efficient purification of these circulating nucleic acids from human plasma, serum, or urine. The extracted products can be used for clinical in vitro detection.

Principle
This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption plate and filter column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10 Mm Tris, pH 8.0).

Advantages
• High yield - most optimal process, free DNA (>50bp) can be obtained to the maximum extent
• High concentration - low elution volume, ensuring high nucleic acid concentration
• High purity - low alcohol binding method, completely removing inhibitor and protein pollution
• High recovery - DNA can be recovered at thelevel of PG by silica gel column purification

Please contact us at for specific academic pricing.