HiPure Total RNA Kit

HiPure Total RNA Kit

Catalog Number:
NAPK357136ANG
Mfr. No.:
IVD4121
Price:
$403
  • Size:
    50 preps
    Quantity:
    Add to Cart:
      • Overview
        • This kit is used for extracting total RNA from non-cell/low cell content biological samples such as body fluid, serum, plasma, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.

          Principle
          This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, RNA is released into the lysate. Transfer to an adsorption plate and filter column. RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, RNA is finally eluted with low-salt buffer.

          Advantages
          • Efficient DNA removal - one step RNA extraction can effectively remove genomic DNA
          • High quality - one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
          • Fast - the whole extraction only takes 15-25 minutes
          • Nontoxic - no toxic phenol chloroform extraction is required in the extraction

          Please contact us at for specific academic pricing.

      • Properties
        • Details
          Purification method: Mini spin column
          Purification technology: Silica technology
          Process method: Manual (centrifugation or vacuum)
          Sample type: Clinical tissues, cells, lymphocytes
          Sample amount: Tissue: <20 mg
          Cells: <5 x 10^6
          Storage
          This kit is shipped and stored at room temperature and is valid for 12 months.

          * For Research Use Only

      • Applications
        • Application
          RT-PCR, cDNA synthesis, second generation sequencing
          Application Description
          Isolation total RNA(miRNA)from tissue, cell using two columns and DNase plus reagent

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