-
-
Overview
-
This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from small volume of blood, tissue and dry blood spots.
Principle
This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mmTris, pH9.0, 0.5mm EDTA).
Advantages
• Fast - several samples can be extracted in 20 minutes (after digestion)
• High purity - purified DNA can be directly used in various downstream applications
• High recovery - DNA can be recovered at the level of PG
• Good repeatability - silica technology can obtain ideal results every timePlease contact us at for specific academic pricing.
-
- Properties
- Applications
-
Overview