HiPure DNA Micro Kit

HiPure DNA Micro Kit

Catalog Number:
NAPK357138ANG
Mfr. No.:
D312502; D312503
Price:
$249
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      • Overview
        • This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from small volume of blood, tissue and dry blood spots.

          Principle
          This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mmTris, pH9.0, 0.5mm EDTA).

          Advantages
          • Fast - several samples can be extracted in 20 minutes (after digestion)
          • High purity - purified DNA can be directly used in various downstream applications
          • High recovery - DNA can be recovered at the level of PG
          • Good repeatability - silica technology can obtain ideal results every time

          Please contact us at for specific academic pricing.

      • Properties
        • Details
          Purification method: Mini spin column
          Purification technology: Silica technology
          Process method: Manual (centrifugation or vacuum)
          Sample type: Animal tissues, blood spots, swabs and various forensic samples
          Sample amount: Blood:1-10μl, Tissue:<2mg
          Storage
          RNase A and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

          * For Research Use Only

      • Applications
        • Application
          PCR, southern bolt and virus detection, etc.
          Application Description
          Isolation total DNA from 1-10μl blood, <2mg tissue and other samples

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