Name: HiPure Tissue DNA Kit
Price: 224.00 USD

- Overview
  - This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from tissue and culture cells.
    
    Principle
    This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while proteinis not adsorbed and is removed with filtration. After washing proteins andother impurities, Nucleic acid was finally eluted with low-salt buffer (10mmTris, pH9.0, 0.5mm EDTA).
    
    Advantages
    • Good repeatability - suitable for extracting high-yield DNA from different types of tissue samples
    • High purity - can be used for downstream applications such as multiplex and quantitative PCR
    • Fast - simplified process, extracting several samples in 20 minutes (after digestion)
    • Safety - no phenol orchloroform extraction, no alcohol precipitation
    
    Please contact us at for specific academic pricing.
- Properties
  - Details
    
    Purification method: Mini spin column
    Purification technology: Silica technology
    Process method: Manual (centrifugation or vacuum)
    Sample type: Animal tissue or cultured cells
    Sample amount: Animal tissue : 1-30mg, Cultured cells : <5 x 10^6
    
    Storage
    
    RNase A and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
    
    * For Research Use Only
- Applications
  - Application
    
    PCR, southern bolt and virus detection, etc.
    
    Application Description
    
    Isolation total DNA from 30 mg tissue, culture cells, FTA card

Documents

D3121 HiPure Tissue DNA Kit

We Also Recommend

HiPure Total RNA Kit

$199

HiPure Total RNA Kit

$403

MagPure Circulating DNA Rich Maxi Kit

$821

MagPure FFPE DNA Kit

$299