HiPure Stool DNA Kit

This product allows rapid and reliable isolation of high-quality genomic DNA from various stool samples. Up to 100 mg soil samples can be processed in 60 minute. The system combines the reversible nucleic acid binding properties of HiPure matrix with the speed and versatility of spin column technology to eliminate PCR inhibiting compounds such as humic acid from soil samples. Purified DNA issuitable for PCR, restriction digestion, and next-generation sequencing. Thereare no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel.

Principle
Stool sample is homogenized and then treated in a specially formulated buffer containing detergent to lyse bacteria, yeast, and fungal samples. Humic acid, proteins, polysaccharides, and other contaminants are removed using our proprietary Absorber Solution. Binding conditions are then adjusted and the sample is applied to a DNA Mini Column. Two rapid wash steps remove trace contaminants and pure DNA is eluted in low ionic strength buffer. Purified DNA can be directly used in downstream applications without the need for further purification.

Advantages
• High purity - unique adsorbent can completely remove inhibitory factors
• High concentration - maximum extraction of total DNA from stool samples
• High recovery - DNA can be recovered at the level of PG
• Good repeatability - silica technology can obtain ideal results every time

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