HiPure Bacterial DNA Kit

This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA can be purified from bacterial culture, body fluids, food and fermentation.

Principle
This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while proteinis not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mmTris, pH9.0, 0.5mm EDTA). This product carries 0.1-0.2mm acid glass beads, which can improve the wall-breaking effect of lysozyme resistant bacteria, the success rate and DNA yield through physical bead grinding.

Advantages
• Fast - several samples can be extracted in 40 minutes (after digestion)
• High purity - purified DNA can be directly used in various downstream applications
• Good repeatability - silica technology can obtain ideal results every time
• High recovery - DNA can be recovered at the level of PG
• Broad spectrum - it can deal with a variety of bacteria, including Gram-positive bacteria that are difficult to disrupt
• Sufficient components - this kit has carried lysozyme, protease K, RNase A and glass beads

Please contact us at for specific academic pricing.