High Binding 96 well Solid plates

High Binding 96 well Solid plates

Catalog Number:
LC01530778BIO
Mfr. No.:
MTB01F4-HB8; MT01F4-HB8; MTW01F4-HB8
Price:
  • Size:
    Quantity:
    Add to Cart:
      • Overview
        • High Binding 96-Well Solid Plates: Solid format designed for high-performance assays.

          Key features of High Binding 96-Well Plates
          · Hydrophilic High Binding Surface: Allows passive adsorption of proteins with varying hydrophilicity, and is highly selective even at low protein concentrations (< 50 ng/cm²).
          · Binding Capacity: Approximately 400 to 500 ng IgG/cm2.
          · Low Fluorescence Polystyrene: Manufactured with pure high-quality polystyrene, minimizing background noise and improving optical clarity.
          · Wells design: flat bottom.
          · Multiple Formats: Available in breakable strip plates, non-breakable strip plates, and solid plates for flexibility in applications.
          · Colors: Available in clear, white, and black polystyrene.
          · Customizable upper rim colors upon request.
          Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays.
          Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
          · Automation-Compatible: Easily integrated with high-throughput automated systems for liquid handling, washing, and analysis.
          · Alphanumeric Coding: For easy and accurate well identification during experiments.
          · Recommended working volume: of 75 to 300 μl
          · Unit: 50 plates
          · Minimum order: 50 plates
          · Packaging: 25 per pack
          · Ready to use

          Key Benefits:
          · Plates Design:
          The mould design provides optical quality, important to reduce the background signal.
          The rim protects the external face of the bottom from scratches.
          · Improved Washing Efficiency: Radius-edged well bottoms enhance washing, reducing residual materials and improving assay consistency.
          · Reduced Cross-Contamination: Designed to minimize the risk of cross-contamination between wells, ensuring precise sample isolation.
          · Enhanced Imaging: The flat surface provides a clear focal plane, essential for high-quality imaging in microscopy, especially for fluorescence and phase-contrast analysis.
          · Improved Mixing: The flat-bottom design facilitates better sample mixing, ensuring consistent results across assays.

          Please contact us at for specific academic pricing.

          Background

          High binding capacity
          High Binding Capacity Surface is highly selective and shows high affinity towards the adsorption of molecules also when those are present in very small amounts allowing to obtain the maximum sensitivity of the test.
          High Binding Capacity surfaces are used in assays where the adsorbed molecule exceeds (up to 400 ng/cm2) the complementary molecules that have to be detected.

          Furthermore, this surface is highly selective and shows high affinity towards the adsorption of molecules also when those are present in very small amounts (<50 ng/cm2) allowing to obtain the maximum sensitivity of the test.

          · Examples of possible applications for High Binding Capacity surface:
          Protein – Antibody Anti-IgM adsorbed onto solid phase used in IgM capture assays
          Lipo-protein – Rubella antigen adsorbed onto solid phase used in IgG assays
          ELISA (competitive) tests for Steroid Hormones and TSH

          · High Binding Capacity Surfaces main features
          Biomat High Binding Capacity products have the following characteristics:
          Ready to Use
          Manufactured under ISO:9001 guidelines
          All lots are tested for uniformity and reproducibility
          Certificate of Quality is released for every lot
          For Research Use Only

          · Also, High Binding Capacity Products ensures:

          Uniformity
          Microplates show a CV% less than 5 using:

          Human IgG as a coating molecule in an ELISA format, anti Human IgG-HRP as detector and TMB as substrate
          Biotinylated albumin as a coating molecule in an ELISA format, streptavidin-HRP as detector and TMB as substrate
          Storage
          The microplates are stored at room temperature in sealed PE bags.

      • Properties
        • Other Properties
          Material: Polystyrene
          Format: Solid
          Bottom: Flat
          Color: Black, Clear, White
          Surface: High binding capacity
          Binding capacity: ~ 400 ng IgG/cm2
          Format: 400 μl
          Uniformity: CV < 5 %
          Shelf life: 5 years
          Packaging: Qty/cs: 200, Qty/pk: 25
          Minimum order: 50 pcs
          Unit: 50 pcs
          Compatibility: Compatible with most common automation systems
          Other information: Certificate of Quality is realeased for every lot
          Dimensions: SBS standard
          Blocking: Standard
          Storage
          Room temperature

          * For research use only.

      • Applications
        • Application Description
          · ELISA Assays: For competitive and sandwich ELISA, detecting a wide range of targets such as steroid hormones and antibodies.
          · Luminescence Assays (LIA): Optimized for high-sensitivity luminescence detection.
          · Fluorescence Assays (FIA): Ensures strong signal detection with minimal interference.
          · Chemiluminescent Assays (CLIA): Ideal for highly sensitive and quantitative analyses.