Hifair™ V2 Multiplex One Step RT-qPCR Probe Kit (UDG Plus, GC enhancer plus)

This kit is a multiplex quantitative PCR kit based on RNA as template. In the process of the experiment, reverse transcription and quantitative PCR were carried out in the same tube, which simplified the experimental operation and reduced the risk of contamination.
In this kit, the first strand cDNA was efficiently synthesized by heat-resistant HifairTM Reverse Transcriptase and quantitatively amplified by UNICONTM HotStart Taq DNA Polymerase. The kit mainly contains optimized MP buffer, enzymes mix, GC-Enhancer etc. The buffer solution already contains Mg2+ and dNTP. In addition, the factors that can effectively inhibit the non-specific PCR amplification and improve the amplification efficiency of multiple qPCR reactions are added, which can ensure the amplification efficiency and carry out up to multiple amplification reaction. The dUTP/UDG system was added to effectively prevent the risk of aerosol contamination.

Features
• Strong versatility - suitable for one-step RT-qPCR detection of nucleic acids from multiple species such as pathogenic microorganisms, human genomes, and plants;
• High sensitivity: The carefully optimized buffer system improves the detection sensitivity of low-concentration templates up to 250 copies/mL;
• dUTP/UDG anti-pollution system: dUTP/ UDG anti-pollution system is introduced to degrade aerosol pollution of products efficiently, reduce false positive, and ensure authentic results;
• Support fast program: compatible with fast program, 30min can produce results.
• Super stability: Stable performance after 14 days at 37°C, long-distance transport, and 10 freeze-thaw cycles.

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