Hifair™ V Multiplex One Step RT-qPCR Probe Kit (UDG Plus)

Hifair™ V Multiplex One Step RT-qPCR Probe Kit (UDG Plus) is a multiplex quantitative PCR kit using RNA as the template. Reverse transcription and qPCR are performed in a single tube, simplifying the workflow and minimizing contamination risk. The kit uses a heat-stable Hifair™ V Reverse Transcriptase for efficient first-strand cDNA synthesis, and UNICON™ HotStart Taq DNA Polymerase for specific and robust amplification. It includes an optimized MP buffer with Mg²⁺, dNTPs, and additives that enhance specificity and multiplex amplification efficiency.
A dUTP/UDG system is incorporated to eliminate carryover contamination from previous PCR reactions, ensuring reliable results.

Features
• High sensitivity: The carefully optimized buffer system improves the detection sensitivity of low-concentration templates up to 250 copies/mL;
• Super stability: The product has passed real-time stability testing for 18 months;
• dUTP/UDG anti-pollution system: dUTP/ UDG anti-pollution system is introduced to degrade aerosol pollution of products efficiently, reduce false positive, and ensure authentic results;
• Support fast program: compatible with fast program, 30min can produce results.

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