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Klenow Fragment (5 U/μL)

Klenow Fragment (5 U/μL)

Catalog Number:
PER1505883YEA
Mfr. No.:
14458ES70
Price:
$228
  • Size:
    200 U
    Quantity:
    Add to Cart:
    Add to Cart
      • Overview

        • Klenow fragment is a protein hydrolysis product of E. coli DNA polymerase I. It retains the DNA polymerase activity and 3'→5' exonuclease activity of DNA polymerase I, but lacks 5'→3' exonuclease activity. It can be used for random primer DNA labeling and cDNA second chain synthesis.
          Unit Definition:
          One activity unit (U) is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid-insoluble precipitate within 30 minutes at 37°C.

          Components: Klenow Fragment (5 U/μL) (40 μL); 10×Klenow Fragment Buffer (1.25 mL)

          Please contact us at for specific academic pricing.

      • Properties
        • Storage
          Transported by dry ice. Store at -25~-15°C, valid for 2 years.
          Note
          Please operate with lab coats and disposable gloves, for your safety.

          * For Research Use Only

      • Applications
        • Application Description
          Dideoxy DNA sequencing (Sanger method)
          DNA labeling using random primers
          CDNA second strand synthesis

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